26035.蔗糖和aba對鐵皮石斛體細胞胚胎發(fā)生的影響

1、西南交通大學碩士學位論文蔗糖和ABA對鐵皮石斛體細胞胚胎發(fā)生的影響姓名：唐靜儀申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：王萬軍20100501西南交通大學碩士研究生學位論文第Ⅱ頁AbstractSomaticembryogenesiswhichiScomplicatedhasbeenallimportantpathwayfor—studyingplantembryogenesisItisaffectedbysomeouter

2、factorssuchassucrose，ABAandnitrogensource，andregulatedbygenesInthisstudy，theembryoniccalliwasusedastheoriginalmaterials，whichwereinducedbyprotocorm—likebodies(PLBs)ofDendrobiumcandidumwallexLindlTheeffectofsucroseandabsc

3、isicacid(ABA)weretestedontheregulationofsomaticembryosofDendrobiumcandidumWaUexLindlTheembryogeniccalliareculturedundertheconditionsofsolid—liquiddifferentiationmediumfor3，4，5，6weeks，theresultsindicatedthatthecombination

4、ofABAandsucrose(5％and6％)weremostefficientforthedevelopmentofembryogeniccallusAndwiththeincreaseofABAconcentration，embryosdevelopmenttendstobeconsistencygrowingwell，andwiththelOWlevelofsecondarybrowningOntheotherhand，thec

5、ombinationof6％sucroseand076／比mol／LABAyieldsthehiighestlevelofsomaticembryo，themediumofexplantsunitgeneratedanaverageof32individualembryos，embryoproductionrateashighas59％Onthebasisoftheresultsobtainedabove，thetotalprotein

6、patternwastestedwiththeembryoniccallusgrowthonthesoliddifferentiationmediumWefoundthatafter5weeksculturetheproteinexpressionreachesitspeak，andtheproteinbandsaremore；Theproteincontentofthetreatmentof076“MABAandsucrosecomb

7、inationiShigherthanthetreatmentof05弘MABAand095／zMAB八andtherearemoresmallmoleculeproteinbandsParticularlyamolecularweightofabout44KDaproteinbandisstrongexpressionunderthesetreatmentsWefindthatPODandESTenzymeactivityoutsid

8、ethetrainingenvironmentwiththehormoneandchangesinSUcrOSesignificantchangeshavetakenplaceComparedwiththecontrolgroupthedifferentiationmediumwasobtainedaftersomaticembryosPODenzymedeficiencyexistsmoreorlesswiththephenomeno

9、nofenzyme，indicatingthattreatmentgroupDendrobiumsomaticembryosdecreasedrespiration；Incontrast，treatmentESTzymogramgrouprelativecontrolgroupemergedwithanewenzyme，indicatingthatnewgeneexpression，increasethestoragematerialA

10、nalysisofsomaticembryogenesisbyregulatingthenatureofLECgenesandfoundfourkindsofLECprotein，therearetotallysimilarphysicalandchemicalproperties，anditsfunctionaldomainofBioinformaticanalysisfoundthatseveralgenesinplantembry

11、odevelopmentplaysananimpoflantregulatoryrole，whichU三C1andLILgenesplayamajorroleinearlyembryonicdevelopmentLEC2andF1JSCA3genesinembryonicdevelopmentstagesofmaturitycancontrolstorageproteinsynthesisandaccumulation，andmakes