26598.發(fā)根農(nóng)桿菌轉(zhuǎn)化懷地黃突變體鑒定與基因克隆

1、河南師范大學(xué)碩士學(xué)位論文發(fā)根農(nóng)桿菌轉(zhuǎn)化懷地黃突變體鑒定與基因克隆姓名：姚換靈申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：周延清20100501ABSTRACTTheobjectivesofthisstudyaretopropagate，transplant，plantandcharacterizethevariantsofRehmanniaglutinosa，huenchingensis(ChartetSechih)Hsiao(calle

2、dvariantsforshortbelow)HWdevelopedbyAgrobacteriumrhizogenesmediatedtransformationandplanttissueculture，andtocloneitsnewgenefragmentinordertolaythefoundationforitsbiotechnologicalbreedingandgeneresearchl、Establishedtheirp

3、ropagationandtransplantations)rstem，successfullyaccomplishedtheircultivationandcharacterizedthembymeansoftheirmorphologicaltraits，chlorophyllcontent，reducingsugarandtotalsugarcontent，freshtuberweightandtubernumberperplan

4、t，theabilitiestodedifferentiateandredifferentiateandDNAvariationanalysisIntermsofmorphologytheleavesofthevariantsturnupwhilethatof855(CK)droop，andtheleavesofthevariantsarelongerandwiderthanthatof855(CK)thevariantsare髂hig

5、hasorhigherthan85—5(CK)，ofwhichvariantHW7andvariantHW8arethehighest，35cmhighAmong12variantstested，thechlorophyllcontentsof7onesarehigherthanthatof855(CK)，where嬲thatof50nesarelowerthanthatof855(CK)，nletotalsugarcontentsof

6、allthefivevariantstestedarehigherthanthatof855(CK)，butthereducingsugarcontentsoffourvariantstestedaresshighasorhigherthanthatof855(CK)，andthatofoneislowerthanthatof855(CK)Thefreshtuberweightperplantof5variantstestedrange

7、dfrom0465to0742g，ofwhich30nes’freshtuberweightperplantareheavierthanthatof855(CK)andthefreshtuberweightperplantofvariantHW12hasa15％increaseC0111【paredtothatof855(CK)Atthesametime，thetubernumberperplantsofthefivevariantsa

8、leallmorethanthatof855(CK)Foritsabilitiytodedifferentiateandredifferentiate，thecallusinductionpercentageofvariantHW12reached100％equalingtooneCKandmorethantwoCKs，anditsshootdifferentiationpercentagefromitscalluseswasthese

9、condhighestamingthe4CKstestedAttheDNAlevel，onespecificDNAfragmentofabout400bpWasnotamplifiedfromthegenomieDNAof85_5(CK)butthegenomieDNAofvariantHW12byoneSRAPprimercombination(em2／eml7／eml8)2、OnenewgenefragmentanditsownOR