益生菌納豆芽孢桿菌的篩選及液體發(fā)酵工藝條件的研究.pdf

1、華南理工大學(xué)碩士學(xué)位論文益生菌納豆芽孢桿菌的篩選及液體發(fā)酵工藝條件的研究姓名：楊曉斌申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：制糖工程指導(dǎo)教師：林慶生20030501華南艘工大學(xué)工學(xué)碩士學(xué)位蜍文_《l___㈣#_=《Ⅻ《#≈_EjE__=!=E自∞#∞％_≈!#=====_㈣=_≈≈女≈∞≈=目l______㈣illlI__≈s_E==_ABSTRACTProbioticsarethenaturalfeedadditives，whicheliminate

2、thenegativeeffe：ctsof魏。扭一nutritionalfeedadditivesespeciallytheantibioticsandpossessthepropertiesoftlon—pollution、nonleftoverantidiseasedaswellaspromotingthegrowthofanimals。Nowadays，probioticshavebeenwidely駐s馥infeedindust

3、ryBut。fewreportshavebeenpresentedtointroducetheusingofbacillussubtilisnattoastheprobiotieinfeedindustryTostudyinthisfield，thispaperintroducestheresearchofscreenandliquidfermentconditionsforbacillussubtUisnatto，Producingf

4、ibriuotysisactivityexperimentsforthestrainsobtainedfromtwen辭kindsofdifferentvarietallobstersauces。w毒screenedoutsevenstrainsnamedasKTI，KT4，KT7，KT9，KT1鐮KT14andKT17。whichhavehigherfibrinolysisactivitiesAfteridentifyinstest，

5、fourbacillussubtilisnattoes，KT1，KT4，KT9andKT14wereobtainedFurtherstudyresultshaveshownthatKTIandKT9notonlyhavethehigherenduredabilitiestotemperature。pHandantibioticthanKT4andKT14butalsohavethebetterabilitiestestraintheno

6、cuousstrains’growthandtOcoexistentgrowwithprobiotic；蟛B4hasalowlivabilitywiththepHrangefrom2lo3：K鼻14isinferiortotheothersintheabilitiestoenduretemperatureandpH；otherwise，tobecomparedwithKT1，KT9possessesthebetterabilityinp

7、roducingpretense，amylaseandcellulase；Forthisreason，霹弘9wasselectedandstudiedforitsliqaidfermentationastheeximiousstraininthisstudyForliquidfermentationontheflasklevelofthebacillussubtilisnattoKT9。theoptimumcultureconditio

8、nsare：glucose2％，soyapeptone2％，NaCl05％，Na2HP04O6％，NaH2P040。1％，CaCl2002％，MgS0401％，MnS04O01％，pH72—73beforesterilizationed，timeofinoculum16h，inoeulumvolumes6弱，volumesofculturemedium100mL／500mL，temperature30℃androtatespeed180

9、rpm。Analyzingthedynamiccurveofthefermentationundertheseconditions，WeCaRfindthat：duringKt9’sfourgrowthphases，thedelayperiodisveryshortabout23hoursonly；thenext22—23hoursisthelogarithmicgrowthphase。Duringthisperiod，thestrai

10、ngrowsquickly，theOD6soandthemaxcelldryweightalsoincreaserapid|ythepHdropfastfrom72to4，4。Meanwhile，thegtucogeisexpendedvastlywiththestraingrowing，theconsumptionisabout70percentiapreceding20hours；Thetimefromhour2223to48i8t