OsPAD：基于二維凝膠電泳系統(tǒng)的水稻蛋白質(zhì)組數(shù)據(jù)庫.pdf

1、浙江大學(xué)生命科學(xué)院碩士學(xué)位論文OsPAD：基于二維凝膠電泳系統(tǒng)的水稻蛋白質(zhì)組數(shù)據(jù)庫姓名：羅聰申請學(xué)位級別：碩士專業(yè)：生物信息學(xué)指導(dǎo)教師：陳銘20100201AbstractRiceisnotonlyaveryimportantagricultureresource，butalsoamodelplantforbiologicalresearchManystudiesintoricehavebeendoneinthefieldsofphys

2、iologygenomicsandproteomicsTwo—dimensionalpolyacrylamidegelelectrophoresis(2DPAGE)isabletomonitorglobalchangesofproteinexpressionunderdifferentcircumstancesofspecifictissuesThusitisapowerfultechniqueforresearchoffice’Spr

3、oteome2D—PAGEhasbeenawidelyutilizedtechniqueinproteomeHoweverforrice，fewsystemic2D—PAGEdatabasesoronlineresourcesareavailable，oneofwhichisRiceProteomeDatabase(RPD，http：／／gene64dnaaffrcgoip／RPD／)RPDcomprisesinformationabo

4、utproteinsidentifiedon2DPAGEmapsofproteinextractsfromawidevarietyoftissuesandsubcellularcompartmentsofriceNotwithstandingitcontainsalargevolumeofdatagainedbyexperimentalworksuchascalculatedpropertiesofeachprotein，aminoac

5、idsequenceandhomologyproteins，theinformationavailableislimitedduetotherestrictionlofexperimentMerelyabout244％ofthetotalgelspotswereannotatedwithinformationfromNCBIwhichisnotsufficientfortheindepthproteomeanalysisOsPAD(Or

6、yzaSativaProteomeAnnotationDatabase)hasbeendevelopedafteranautomaticannotationprocedurewasdoneItsupportsnotonlydatabasesearchingbutalsoonlineanalysisofusers’submittingdataviaauserfriendlyinterfaceOsPADisasystemicproteome