益生菌對e.colio78在雛雞消化道黏附的影響

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文益生菌對E.coli O78在雛雞消化道黏附的影響姓名：魏玉環(huán)申請學(xué)位級別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：魏萍20090620Abstract iii The Effect of Adhesion with Probiotic on E.coli O78 Indigestive Teact of Chicken Abstract The study was finished by two kind of prob

2、iotics selected from chicken, its aim is to detected the effect of adhesion in E.coli O78 in the digestive of chicken and properties of probiotics in vitro. In these experiments,the bearing capacity of acid and bile sal

3、t of probiotics were firstly detect by it’s survival rate under the different pH values and bile concentration in MRS medium. Secondly, adhesion capacity of chicken intestinal epithelial cells was detected with probiotic

4、s through Gram stain, the adhesion inhibiting capacity of enteropathogenic E.coli in intestinal epithelial cells with probiotics was detected by indirect immunofluorescence method in vitro. Then the vivo study of chicken

5、s was made according to the results in vitro. Sensitive antibiotics was Selected cefoperazonly with intestinal flora of chicks by susceptibility test methods, to set up dysbacteriosis model induced by antibiotics, The ef

6、fects of adhesion ability of probiotics on avian enteropathogenic E.coli O78 in the chicken digestive tract were tested, 1d 150 uninfected chicks were randomly divided into 6 groups of, treatment groups as follows: Group

7、 1:Cefoperazone were drenched with Concentration of 40mg/kg/d succession for three days in order to cleansing intestinal bacteria, taking orally 0.5ml with 2 × 109cfu/ml concentration avian enteropathogenic E.coli O

8、78 in fourth day. Group 2: Remove intestinal bacteria same with group 1, taking orally 0.5ml with 2 × 109cfu/ml concentration avian enteropathogenic E.coli O78 and simultaneous inoculation mixed liquid by equidensit

9、y of Lactobacillus acidophilus and Lactobacillus casei in fourth day. Group 3: Terile iso-osmia salines was drenched 0.5ml succession for three days and take orally 0.5ml with 2 × 109cfu/ml concentration of avian en

10、teropathogenic E.coli O78. Group 4: Drenching succession same with group 3 for three days, then taking orally same with group 2 in fourth day. Group 5: The blank control of group1 and group 2. Group 6: The blank control

11、of group3 and group 4. O78 on the enteron mucosa in craw, stomachus glandularis, duodenum, jejunum, ileum, caecum and rectum was identified and demeshed by the method of Gram stain, biochemical identification, and indire