國人IL-1基因多態(tài)性與Hp感染后胃癌易感性的研究.pdf

1、分類號：R57碩士磺究生學(xué)位論文Y767q4單位代碼學(xué)號1190420022128國人IL一1基因多態(tài)性與Hp感染后胃癌易感性的研究Interleukin一1PolymorphismsAssociatedwiththeRiskofGastricCarcinomainChinesewithHelicobacterpyloriInfection專業(yè)：內(nèi)科學(xué)消化專業(yè)研究生：張穎導(dǎo)師：張志廣主任天津醫(yī)科大學(xué)二00五年五囂查堡墮型查蘭堡主蘭些堡蘭

2、ABSTRACTOBJECTIVE：Toevaluatetheassociationbetweeninterleukin1(IL1)locipolymorphismsandincreasedriskofgastriccarcinomainsamplesfromnorthernChinesepopulationM[ETHODS：Bloodsamplefrom76patientswithgastriccancerand75controlsw

3、ithchronicgastritiswerecollectedGenomicDNAWasextractedandpolymorphismsat一31(CtoT)，511(CtoT)andatintron2(86bpVNTR)ofIL1RNweregenotypedbyPCRCTPPPCR—RFLPandPCRRESULTS：FivekindsofpolymorphismoflL一1RNwergfoundas1／1，1／2，1／3，1／

4、4，2／2andthefrequenciesinpatientswere7763％，1184％，395％，658％and0％，respectivelyHoweverthefrequenciesincontrolswere7733％，1333％，400％，400％andI33％NosignificantdifferenceswereobservedbetweencasesandcontrolsineachgeuotypeThepolymo

5、rphismofIL1B一31allelewasC／C，C／TandT／TThefrequenciesinpatientswere1081％，4865％，4054％andthefrequenciesincontrolswere2838％，4865％and2297％IL一1B一31T／TCalTierswereatanincreaseriskofgastriccancer謝t11a11oddsratioof4632(95％CI，16901

6、2700)IL一1B511alleleswereC／CC／TandT／TThefrequenciesinpatientswere1600％，6000％，2400％andthefrequenciesincontrolswere2027％，5270％and2703％NosignificantdifferenceswereobservedbetweencasesandcontrolsineachgenotypeCONCLUSION：InChi

7、nesepopulation，thepolymorphismofIL1B31allelesmaybeassociatedwiththesusceptibilityofgastriccancerHowevernoevidencewasfoundtosupportthatthepolymorphismsofIL1RNandIL1B511alleleshadrelationshipwitllgastriccancerKEYWORDS：np；I