26869.不同產(chǎn)地娑羅子主要成分分析比較研究

1、甘肅農(nóng)業(yè)大學(xué)碩士學(xué)位論文不同產(chǎn)地娑羅子主要成分分析比較研究姓名：邊靜靜申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：野生動(dòng)植物保護(hù)與利用指導(dǎo)教師：趙樺劉發(fā)央20100601甘肅農(nóng)業(yè)大學(xué)2010屆碩士學(xué)位畢業(yè)論文SummaryTheOilobtainedfromSemenAesculiofthedifferentareaswereextractedbySoxhletextractionrespectivelytheoilcontentweredetermine

2、dTheFat—solubleextractedfromTiantaishanHanzhong，identifiedanddeterminedbygaschromatography—massadoptedingaschromatographyThepolysaccharideofSemenAesculiWasextractedbyhotwaterproteinWasremovedbyTrichloroaceticacid，thecont

3、entofthepolysaccharidewasdeterminedbyphenolsulphuricacidmethodThescavengingactivitiesofthepolysaccharidefromSemenAesculionhydroxylradical(‘OH)，hydrogenperoxide(H202)and1，1Diphenyl2一picrylhydrazyl(DPPH‘)wereinvestigatedby

4、modifiedchemicalsystemsAntimicrobialactivitytestingusedthemethodoftraceliquiddilutionThecontentsofaescinAandaeseinBinSemenAesculiofdifferentareasweredeterminedbyhighperformanceliquidchromatography(HPLC)usingCls(46mmxl50m

5、m，51am)colurrmwithacetonitrile02％phosphoricacid(37：63)asmobilephaseataflowrateof10ml／min，anddetectionwavelengthof220nnl，columntemperatureof25℃1ThecontentoftheoilfromSemenAesculiis040％“137％Twocompositionsweredetermined，th

6、enonpolaritycompoundsandpolaritycompounds32componentswereidentifiedfromthenon—polaritycompounds，themaincomponentswereNonacosane(1507％)，Hentdacontane(1085％)，Heptacosane(700％)，Pentacosane(699％)，Tricosane(627％)，Tetraeosane(

7、559％)，Hexacosane(574％)，Octacosane(512％)，etc22componentswereidentifiedfromthepolaritycompounds，including6unsaturatedfattyacids，9Octadecenoicacid(421％)，9Octadecenoicacid(343％)，9，12Octadecadienoieacid(096)，9，11Octadecadieno

8、ieacid(010％)，9Hexadecenoicacid(015％)，15Tetracosenoicacid(01O％)In15saturatedfattyacids，themaincomponentswereHexadecanoicacid(6594％)，Octadecanoicacid(1157％)，Tetradecanoicacid(503％)，Eicosanoicacid(166％)Decanoicacid(114％)，Do

9、decanoicacid(1O1％)，Tetracosanoicacid(083％)，ete2neaveragecontentofpolysaccharidefromSemenAesculiis375％ThescavengingeffecttothethreefreeradicalsofthepolysaccharidefromSemenAesculiislowercomparedwithVeHoweverthescavengingef

10、fectofcrudepolysaccharideisstrongerthanpurepolysaccharideThetwopolysaccharideshaddefiniteantimicrobialeffectstothefivebacteriaandfungisamples，andtheantimicrobialeffectsofcrudepolysaccharideisstrongerthanthepurepolysaccha