紅冬孢酶母中AHL降解酶的分離純化.pdf

1、河北工業(yè)大學(xué)碩士學(xué)位論文紅冬孢酶母中AHL降解酶的分離純化姓名：羅利龍申請學(xué)位級別：碩士專業(yè)：生物化工指導(dǎo)教師：宋水山20100501河北工業(yè)大學(xué)碩士學(xué)位論文 iii THE SEPARATION AND PURIFICATION OF AHL-DEGRADATION ENZYME IN RHODOSPORIDIUM TORULOIDES ABSTRACT Quorum-Sensing(QS), which regulates ge

2、ne expression depending on population density, is universal in bacteria. QS is involved in the regulation of virulent factor and pathogenic gene expression, and plays a key role in causing plant disease by plant bacteria

3、l pathogens. Hense, targeting the bacterial QS seem to be a promising approach for control the disease caused by QS-mediated bacterial pathogens. N-acyl homoserine lactone (AHL) acts as a key singnal in bacterial Quorum-

4、Sensing. Several groups of AHL-degradation enzymes have recently been identified in a rang of living organisms including bacteria and eukaryotes. Expression of these genes encoding AHL-dagrading enzyme in AHL-dependent p

5、athogens and plants efficiently quench the acceleration of QS signal and blocks pathogenic infection.Discovery of these novel quorum quenching enzymes has not only provided a promising means to control bacterial infectio

6、ns,but also presents new challenges to investigate their roles in host organisms and their potential impact on ecosystems. Qiu jian et al screened a yeast which can be able to degrade AHL and was identified as Rhodospori

7、dium toruloides. The discovery riched the quorum quenching enzyme.In this paper we success in separation and purification of this enzyme. The R. toruloides was cultured in MacConkey medium and then we collected the bacte