轉(zhuǎn)OsSec27p和OsARHI基因煙草分子鑒定和不同鐵處理的生理指標(biāo)檢測(cè).pdf

1、湖南農(nóng)業(yè)大學(xué)碩士學(xué)位論文轉(zhuǎn)OsSec27p和OsARHI基因煙草分子鑒定和不同鐵處理的生理指標(biāo)檢測(cè)姓名：李淑榮申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：饒力群印莉萍20071201AbstractOsSec2Zp、OsARHIaretwogenesof16upregulatedgenesrelatedvesicletraffickingresponsetoirondeficiencyinriceOurlaboratoryha

2、vebeenblastedandanalysedOsSec2Zp、OsARHIandaequkedOsSec27p&略是≠髫秘|lengthsequenceTheyconstructplantexpressionvectorsCAMBIAl302一OsSec27p，pCAMBIAl302一OsARHIseparately，andhavebeentransformedintotobacco，andacquiredToandTIgermso

3、fOsSec27p、OsARHIthetwotransgenetictobacco。Inthisthesis，wehavejustbasedonthepreviousresultinourlab，analyzingandidentifyingtheTlgermoftransgenetictobaccointhemolecularandphysicallevels。Ourexperimentjustbasedonthepreviousre

4、sultfromthreelevels，resistantselect，PCRdetectioningenelevelfandGFPdetectioninproteinlevel。Weusedshapeobservation，determiningthepHvalueandtheIroncontenttophysicalstandarddetecfionTheresultsofourexperimentshowthat：thetarge

5、tgenefragmenthasbeenassuredlytransformedintotobacco，andthegenefragmenthasnotbeendeletedduringthegrowthoftobacco。Byusinggreenfluorescencemonitor，wefoundthattheexpressionofGFPishigherinthetransgenetictobaccespondedtoironde

6、ficiencyIdentifyingthetransgenetictobacorootsandleaves，thedatashowedthatOntheconditionofirondeficiencythetoleranceoftransgenetictobacco、whowith03ARHlandOsSec27pwasenhance，androotscouldabsorbironefficientlyBesides，theabil

7、ityofirontransportintobaccowasimprovedByusingobservationstatisticsandstudyingthegrowthoftrausgenetictobacco。wefoundthatinresponse幻irondeficiency，thenumberoftobaccolateralrootincreasedapparentlyFurthermorebydeterminingthe

8、pHvalueoftheculturewecanpredictthattherootoftransgenetictobaccowouldexcretesomeacidsubstancewhichwouldchangetheacidityofcultureSOastoaffecttheabortionrateofionintheenvironmentKeyword：transgenetictobacco，OsSec2Zp，OsARHI，P