轉OsSec27p和OsARHI基因煙草分子鑒定和不同鐵處理的生理指標檢測.pdf

1、湖南農(nóng)業(yè)大學碩士學位論文轉OsSec27p和OsARHI基因煙草分子鑒定和不同鐵處理的生理指標檢測姓名：李淑榮申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：饒力群印莉萍20071201AbstractOsSec2Zp、OsARHIaretwogenesof16upregulatedgenesrelatedvesicletraffickingresponsetoirondeficiencyinriceOurlaboratoryha

2、vebeenblastedandanalysedOsSec2Zp、OsARHIandaequkedOsSec27p&略是≠髫秘|lengthsequenceTheyconstructplantexpressionvectorsCAMBIAl302一OsSec27p，pCAMBIAl302一OsARHIseparately，andhavebeentransformedintotobacco，andacquiredToandTIgermso

3、fOsSec27p、OsARHIthetwotransgenetictobacco。Inthisthesis，wehavejustbasedonthepreviousresultinourlab，analyzingandidentifyingtheTlgermoftransgenetictobaccointhemolecularandphysicallevels。Ourexperimentjustbasedonthepreviousre

4、sultfromthreelevels，resistantselect，PCRdetectioningenelevelfandGFPdetectioninproteinlevel。Weusedshapeobservation，determiningthepHvalueandtheIroncontenttophysicalstandarddetecfionTheresultsofourexperimentshowthat：thetarge

5、tgenefragmenthasbeenassuredlytransformedintotobacco，andthegenefragmenthasnotbeendeletedduringthegrowthoftobacco。Byusinggreenfluorescencemonitor，wefoundthattheexpressionofGFPishigherinthetransgenetictobaccespondedtoironde

6、ficiencyIdentifyingthetransgenetictobacorootsandleaves，thedatashowedthatOntheconditionofirondeficiencythetoleranceoftransgenetictobacco、whowith03ARHlandOsSec27pwasenhance，androotscouldabsorbironefficientlyBesides，theabil

7、ityofirontransportintobaccowasimprovedByusingobservationstatisticsandstudyingthegrowthoftrausgenetictobacco。wefoundthatinresponse幻irondeficiency，thenumberoftobaccolateralrootincreasedapparentlyFurthermorebydeterminingthe

8、pHvalueoftheculturewecanpredictthattherootoftransgenetictobaccowouldexcretesomeacidsubstancewhichwouldchangetheacidityofcultureSOastoaffecttheabortionrateofionintheenvironmentKeyword：transgenetictobacco，OsSec2Zp，OsARHI，P