細(xì)胞色素b基因RNA干擾載體的構(gòu)建及擬南芥的遺傳轉(zhuǎn)化.pdf

1、華中科技大學(xué)碩士學(xué)位論文細(xì)胞色素b基因RNA干擾載體的構(gòu)建及擬南芥的遺傳轉(zhuǎn)化姓名：周璇申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物醫(yī)學(xué)工程指導(dǎo)教師：何光源楊廣笑20110108華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文IIAbstractInplantcellsexceptnucleuschloplastmitochondriaalsocontaingeicmaterialDNA.WiththedevelopmentDNAsequencingte

2、chnologythenumberoftheplantsspecieswhosenuclearchloplastmitochondrialgenomesequencingprojectshavebeencompletedissteadlyincreasingrecently.Studieonthegenomestructuregeneinfmationinnucleuschloplastsmitochondriabythecombina

3、tionmethodofbioinfmaticsbiologicalexperimentscouldprovideimptantreferenceonthegeicrelationshipamongdifferentspeciestheiginofplantspeciestheirevolution.ArtificialmicrNAs(amiRNA)genesilencingtechnologyisconsideredasaeffici

4、entmethodfstudyingthemechanismofcodinationgeneexpressionbetweennuclearganellargenomes.Cytochromeb(cob)isoneoftheimptantcomponentinrespiratychainwhichisencodedbothbynuclearmitochondrialgenomeeachwithonlyonecopyinArabidops

5、isthaliana.CobgeneknockdownbyartificialmicrNAsgenesilencingtechnologyshouldhelptorevealthemechanismofcodinatedregulationofcobgeneexpressionbetweennuclearganellargenomes.Inthepresentresearchcobsilencingvectsfnucleusgenome

6、wereconstructedbyamiRNAtechnologyweretransfmedintoA.thalianaviaAgrobacteriummediatedmethod.Themainresultsareasfollows:1)ThroughanalyzingthefeaturesofthenaturalmiRNAthecidatesprovidedbyWMD3amicrNAofthenucleuscobgenewasdes

7、igned.2)NucleuscobsilencingvectswasconstructedusingthetemplatessynthesedbyoverlappingPCRmethod.3)NuclearcobsilencingvectsweretransfmedintoA.thalianaviaAgrobacteriummediatedmethod.InconclusionA.thalianancobsilencingvectsw

8、assuccessfullyconstructed.Theresultsofpresentresearchhaspavedthewayfstudyingthemechanismofcodinatedregulationofcobgeneexpressionbetweennuclearganellargenomes.Keywds：Arabidopsisthaliana.CytochromebRNAinterferenceArtificia