ENOPH1在急性缺血性腦卒中血腦屏障內皮細胞凋亡中的功能及機制研究.pdf

1、分類號：R743.3密級：公開UDC：616.8編號：2014214681碩士學位論文碩士學位論文ENOPH1ENOPH1在急性缺血性腦卒中血腦屏障內皮細胞凋亡中在急性缺血性腦卒中血腦屏障內皮細胞凋亡中的功能及機制研究的功能及機制研究CerebralMicrovularEndothelialCellApoptosisafterIschemia:RoleofEnolasePhosphatase1ActivationAciReductone

2、Dioxygenase1Translocation研究生：王婷導師：劉文蘭教授申請學位級別：醫(yī)學碩士年級：二O一四級學科專業(yè)：神經病學研究方向：早期血腦屏障損傷論文提交日期：二O一七年五月論文答辯日期：二O一七年五月學位類型：臨床專業(yè)型學位授予單位：廣州醫(yī)科大學答辯委員會主席：評閱人：汪志宏教授金新春教授二O一七年五月目錄中文摘要......................................................

3、........1ABSTRACT..............................................................2中英文名詞對照與縮寫..................................................4前言..................................................................51.實驗材料與方法.

4、.....................................................71.1實驗材料.......................................................71.1.1實驗動物及細胞株........................................71.1.2抗體..........................................

5、..........71.1.3試劑及試劑盒............................................71.2研究方法.......................................................81.2.1構建大鼠MCAO模型.......................................81.2.2分離腦微血管并取材.....................

6、.................81.2.3細胞培養(yǎng)................................................91.2.4OGD模型制備.............................................91.2.5乳酸脫氫酶釋放檢測法...................................101.2.6ENOPH1siRNA轉染方法..............

7、.....................101.2.7ENOPH1CRISPRActivationPlas轉染方法...............101.2.8單層內皮細胞BBB模型...................................111.2.9內皮細胞通透性實驗.....................................111.2.10細胞內活性氧（ROS）產生的檢測.................

8、........121.2.11Westernblot分析各種蛋白表達..........................121.2.12免疫共沉淀............................................131.2.13免疫細胞化學..........................................131.2.14實時熒光定量RTPCR...........................

9、.........141.2.15TUNEL檢測細胞凋亡.....................................141.2.16統(tǒng)計學分析............................................152.結果...............................................................162.1急性缺血性腦卒中早期腦微血管組織ENOPH1表達