斑馬魚視黃醛脫氫酶2基因（raldh2）的啟動子活性分析及其受視黃酸調(diào)控的反饋抑制機制.pdf

1、南京師范大學(xué)碩士學(xué)位論文斑馬魚視黃醛脫氫酶2基因（raldh2）的啟動子活性分析及其受視黃酸調(diào)控的反饋抑制機制姓名：張媚申請學(xué)位級別：碩士專業(yè)：遺傳學(xué)指導(dǎo)教師：許曉風(fēng)趙慶順20080501望壹室塹蔓奎堂堡圭蘭些望莖整塑●__l__●_●____●___l____●●________●I__●_●_●_●_l__l____●___I_______。。。。。。’’———————一一一一AnalysisofZebrafishraldh2Pro

2、moterrevealingthattherepressionofraldh2byRAIsMediatedbyRAREsintheFirstIntronABSTRACTRALDI12isthemainRetinoicacid∞qsynthesizingenzymeduringdevelopmentandisthemostwidelyexpressedinundifferentiatedsomites，limbbudsandvarious

3、organsTheexpressionofRALDH2geneisrepressedbyRAHoweverthemechanismofthisrepressionisstillunknownPreviousstudiessuggestthatraldh2isadirectRAresponsegene，andraldh2expressionisdecreasedwithRAtreatmentAnalysesoftheraldh2promo

4、terincelltransfeetionsprovedthatRAdirectlyrepressesitsactivityThesedatademonstratethatthereisafeedbackmechanismregulatingproductionofRANeverthelessthepromoterthatWaschosentod0activityassaydidnotshowniceactivityPerforming

5、bioinformaticsanalysiSwedidnotfindanyrctinoicacidresponseelements(RAREs)inthebasicpromoterabout27kbupstreamofthetranscriptionstartsiteWhereas，somepotentialcandidateRAREsarefoundinthefirstinlxonInthisstudywemadesomepromot

6、erconstructscontainingthebasicpromoterandthefragmentsfromtheintron1，whicharelocatedfrom27kbupstreamofthetranscriptionstartsitetoabout2kbdownstreamandfrom13573to15115Byperformingdualluciferaseassayofthefragmentscontaining

7、thoseRAREs，wefoundthatRAinducesastronginhibitionoftheexpressionofraldh2TheseresultssuggestthatthepotentailRAREswhicharclocatedintheintron1from13573to15115，mediatetherepressiveeffectofRAAdditionallywefoundthatthefeedbackl