中藥海藻海蒿子多糖的分離與鑒定及其抗腫瘤活性初步研究.pdf

1、分婁號UDC窳級⑧研究生學(xué)位論文墓拭勝擅活蛙麴生盟塞研劃擻名——劐越一——指撒讎煎簧堡透室鰱量王籃垂數(shù)自請糊綴別亟主刳喘稱壘夔堂論文答辯日期；!!箜笙!旦!一旦學(xué)位授ffl期!叢；疊l壁—且，中國海洋大學(xué)Preparation，identificationandprimaryantitumorbioactivityscreeningofpoIysaccharidesfromtheGhineseMadeciBe—SargassumPa||

2、idumAbstract：SargassumpallidumisusedforChinesetraditionalmedicineforhundredsofyearsIthasbeenknowntoproducedifferentpolysaccharides，namelyfucoidans，alginatesandlaminaransSeveralbiologicalactivitieshavebeenattributedtothef

3、ucoidans：anticoagulant，antithrombotic，anti—inflammatoryantitumoral，antraceptive，andantiviralFucoidans(SPC60(precipitated晰th60％ethan01)，SPC70，SPH60andSPH70)werefractionatedprecipitationfromthewaterextraction，theresiduewas

4、extractedwithNa2C03solutionandproducedSPA(aiginate)ThecharacteristicdifferencesbetweenSPCandSPHwereinvestigated，andtheresultsindicatedthatthedifferentextractionandfractionationwouldproducedifferentfucoidansCrudefucoidans

5、werepurifiedandfractionatedtopolysaccharides(P3andP4)byanionexchangecolumn(QSepharoseFastFlow)andchromatographycolumn(SephadexG200andSepharoseCL6B)ThedifferencesbetweenSPH70P3andSPH70P4werestudiedandrevealedthatcrudefuco

6、idancanseparateintoheterogeneousfucoidansTotalcarbohydratescontents，IIPGPC，Monosaccharidescompositions，F(xiàn)T11LMethylationandPeriodateoxidationanalysisconfirmedthecharacteristicofSPHTOP4：contained8215％totalcarbohydrates，163

7、8％sulfate，342％uroIlicacid；molecularweightis410kD；carbohydratecompositionsareRha，F(xiàn)uc，Xyl，Man，GluandGalthemolarratiois063：401：091：358：100：258；hadabsorbanceat840cm’1inIR；thelinkageis：Thefucoidanconstituentismainlycomposedof

8、1—24and1—3linkedfucopyranosyl，1—2，34and1—34，6一linkedmarmopyranoseand1—36一linkedgalactopyranoseunits，theglucopyranoseisonthebranchesThesulfationpattemwithsubstitutionprincipallyatposition2and／orposition3offucopyranosylWea