燈盞細(xì)辛提取物對(duì)高壓狀態(tài)下視網(wǎng)膜保護(hù)作用的基因調(diào)控研究.pdf

1、成都中醫(yī)藥大學(xué)博士學(xué)位論文燈盞細(xì)辛提取物對(duì)高壓狀態(tài)下視網(wǎng)膜保護(hù)作用的基因調(diào)控研究姓名：曾潔萍申請(qǐng)學(xué)位級(jí)別：博士專(zhuān)業(yè)：中醫(yī)五官科學(xué)(眼科)指導(dǎo)教師：段俊國(guó)2008-05ABSTRACT Objective: 1. To observe the protection of DSX on gene regulation of rat RGCs cultured in vitro with high pressure. 2. To observ

2、e the protection of DSX on gene regulation of the rat retinal with chronic, moderately elevated intr aocular pressure (IOP). 3. Analyze the mechanism of protection of DSX for rat retina. Method: Setting up RGCs high pres

3、sure model cultured in vitro with the condition of 80mmHg for 4 hours, then using gene array technology to study the regulation of gene express of rat RGCs on the variation of pressure. 2. Adding DSX into RGCs cultured w

4、ith high pressure, then using gene array technology to observe the effect of DSX on the regulation the gene express of rat RGCs. 3. Building the model of chronicity, moderately elevated intraocular pressure by the method

5、 of Akira’s, then using gene array technology to study the regulation to gene express of rat retina. 4. Giving DSX orally on IOP rat for one month, then using gene array to observe the effect of DSX on the regulation on

6、the gene express of retina. Results: 1.There was 108 genes had differential expression between the model group and the control group of RGCs. such as apoptosis gene NGFR, PAWR, S100B and ESTs was up-regulation among，othe

7、r genes hand changed including the transmission of nerve impulse, synaptic transmission, cation channel activity, cell-cell signaling, cytoskeletal protein binding and extracellular matrix. 2. There was 51 genes had diff

8、erential expression between the test group and the model group of RGCs. apoptosis inhibiting genes Bcl2a1 and Spp1 were up-regulation and apoptosis gene NGFR was down-regulation among them, other response to external bio

9、tic stimulus genes(4) and ATPase activity genes (3) were up-regulation. 3.There was 41 genes had differential expression between the positive control group and the model group of RGCs. apoptosis inhibiting genes Hmox1 wa

10、s up-regulation and apoptosis gene S100B and NGFR was down-regulation among.4. There was 22 genes had differential expression between the model group and the blank group of retinal apoptosis genes NGFR was up-regulation

11、and CRYBB1 was down-regulation among. 5. There was 17 genes had differential expression between the test group and the model group of retinal apoptosis genes NGFR was down-regulation and CRYBB3, CRYGD and CRYBB2 were up-

12、regulation among. Conclusion:1.The pressure could down-regulate the genes such as glutamic acid metabolism or transporter, so made high concentration of Glu, then stimulate Ca2+ flowing into intra-cellular， high concentr

13、ation of Ca2+ induced signal transduction system producing cascade reaction, apoptosis gene was up-regulated to make RGCs apoptosis. 2. DSX can improve energy metabolism, clean Glu for RGCs, then lessen Ca2+ inflow，avoid

14、ing Ca2+ overloading intra-cellular，blocking up cell signal transduction pathway, making apoptosis genes NGFR down regulation.3. DSX play the role as the protectant of retina by up-regulating apoptosis inhibiting genes B

15、cl2a1 and Spp1 and several genes about crystalline. 4.The protection of DSX for retina is multi- target，complex mechanism，and identify. Key words: RGCs culture; protection of optic nerve; gene array; apoptosis gene; apop