營養(yǎng)限制與補(bǔ)償對蒙古羔羊嫩度相關(guān)基因mRNA表達(dá)量的影響.pdf

1、分類號Q344.13UDC636學(xué)校代碼10129學(xué)號09209005營養(yǎng)限制與補(bǔ)償對蒙古羔羊嫩度相關(guān)基因mRNA表達(dá)量的影響EffectofUndernutritionCompensationontheTendernessRelatedGenemRNAExpressioninSheep申請人：黃雅娟學(xué)科門類：工學(xué)學(xué)科專業(yè)：食品科學(xué)研究方向：食品質(zhì)量與安全指導(dǎo)教師：靳燁教授高愛武副教授論文提交日期：二〇一二年六月EffectofUnde

2、rnutritionCompensationontheTendernessRelatedGenemRNAExpressioninSheepAbstractThisexperimentwasconductedtoresearchtheeffectsofdifferentdietaryenergyproteinratiorestrictionfeedcompensationonthemeatqualitytendernessrelevant

3、geneexpressioninlambEightyWuZhuMuQinlambs(averagebodyweight14.72kg)wereeddividedinto4groups.Thefeedingtriallasted150daysincludes60daysoffeedrestriction90daysoffeedcompensation.Thenutritionlevelofthedietinfeedrestrictionp

4、eriod(060d)wereME:10.88MJkgCP:10%frestrictedgroupⅠ(RGⅠ)ME:9.41MJkgCP:10%frestrictedgroupⅡ(RGⅡ)ME:8.62MJkgCP:5.7%frestrictedgroupⅢ(RGⅢ)ME:10.88MJkgCP:15%fcontrolgroup(CG).Experimentaldietswereadlibitumwaterwereavailabledu

5、ringtheentireexperimentalperiod.Duringnutritioncompensatyperiod(60150days)alllambswereofferedthesamediet.Theexperimentalresultsshowed:NutrientlimitationpromotedcalpainfamilyCalpain1Calpain2CASTgeneexpressionthroughthecom

6、pensatygrowthallgenesexpressionreturntonmallevelexceptCalpain2gene.LowproteinnutritionrestrictioninhibitedCathepsinBCathepsinDexpressionbutpromoteCathepsinLexpression.LowenergylevelsofnutritionrestrictiononCathepsinBCath

7、epsinDCathepsinLexpressionlevelhavelittleeffects.ProteinenergydoublelimitslevelonCathepsinBCathepsinDexpressionwithoutnegativeeffectsbutonCathepsinLexpressionhavenegativeeffect.ThelaterperiodCathepsinBCathepsinnutritionc

8、ompensationsonCathepsinLexpressionhavenegativeeffectswhilepromotingtheCathepsinDexpression.Nutritionrestrictiononpostmtem45minsingroupRG3pHhaveavariationinfluence.Attheendofthelimitperiodonpostmtem45mins24hlambsinlongiss

9、imusdsiofpHmanifestationsofRG3wasthehighest.Attheendofthecompensationperiodpostmtem45minsingroupRG2afterpHwassignificantlyhigherthantheotherthreegroups.Inthepostmtem24hallgroupspHchangeshavenodifference.Nutritionrestrict

10、ioncompensationonmusclewaterholdingcapacityhavenoeffect.AttheendofthelimitperiodgroupRG3shearfcevaluessignificantlylowerthantheotherthreegroups.AttheendofcompensationperiodRG1groupRG2groupweresignificantlyhigherthantheco