遠(yuǎn)志與厚樸配伍對小鼠Cajal間質(zhì)細(xì)胞的影響.pdf

1、成都中醫(yī)藥大學(xué)碩士學(xué)位論文遠(yuǎn)志與厚樸配伍對小鼠Cajal間質(zhì)細(xì)胞的影響姓名：劉莉娜申請學(xué)位級別：碩士專業(yè)：中藥學(xué)指導(dǎo)教師：王建2010-04ABSTRACT Objective: Study from the cellular level and Magnolia Combination Polygala by vitro gastric interstitial cells of Cajal(GICC) growth in mouse

2、 and its related active substances． Methods: ①By Koh’method, we improved the culture of GICC, using MTT colorimetric test (MTT) to study the control group, group Polygala, Magnolia Group, Polygala and Magnolia 1:1,1:2,1:

3、3compatibility group of GICC to optimal ratio. ②Using Colorimetric, we compared the control group, Polygala serum group, Magnolia serum group, Polygala and Magnolia 1:2compatibility serum group of GICC with Lactate dehyd

4、rogenase (LDH), and calcium (Ca2+) activity of mechanism. ③By the Pi method, we studied the drug-containing serum on GICC of ATPase(ATP enzyme). ④Using thiobarbituric acid (TBA) method, we studied the drug-containing ser

5、um group on GICC of malondialdehyde (MDA). ⑤By Griess method, we studied the drug-containing serum of GICC medium of nitric oxide (NO) levels, and investigated effect in different groups of GICC. Results: ①Decoction of d