survivin反義寡核苷酸對人膽管癌裸鼠移植瘤及c-myc和cyclinD1表達的影響.pdf

1、中南大學碩士學位論文survivin反義寡核苷酸對人膽管癌裸鼠移植瘤及cmyc和cyclinD1表達的影響姓名：魏偉申請學位級別：碩士專業(yè)：外科學（普外）指導教師：湯恢煥20050501Abstractobjective：1Toinvestigatetheeffectsofsurvivinantisenseoligodeoxynucleotides(ASODN)onthegrowthoftransplantedhumancholangi

2、carcinomainnudemice2ToinvestigatetheeffectsofsurvivinASODNontheexpressionlevelofsurvivin，cmycandcyclinD1proteinMethods：EstablishmousetransplantedtumourmodelQBC939celllinewere竭ectedSubcutaneouslyFortyeighthoursafterinject

3、ion，SurvivinASODNsenseoligodeoxynucleotides(SODN)andO9％sodiumchloridesolutionwereinjectedsubcutaneouslyintonudemiceofASODNgroup，SODNgroupandcontrolgroup，respectivelyThelengthandwidthoftumornodulesweremeasuredThetumorvolu

4、meandweightweremeasured，HEstaining，theexpressionofsurvivin，c—mycandcyclinD1weredetectedbyimmunohistochemistryImageanalysissystemwasadoptedtoevaluationtheirgreyscaleResults：Transplantedhumancholangicarcinomaweredevelopedi

5、n23nudemiceexceptoneinASODNgroupGrowthoftumorwassignificantlyinhibitedinASODNgroupascomparedwitllthatincontrolgroupThetumorvolumeandweightinASODNgroupwerelessthancontrolgroupsignificantly(PO01)Thegreyscaleofsurvivin，cmyc

6、andcyclinD1inASODNgroupweresignificantlyhigherthancontrolgroup(PO01)，whereasinSODNgrouptherewerenosignificantdifferenceConclusion：1Theexpressionrateofsurvivin，C—mycandcyclinD1proteinareveryhighintransplantedhumancholangi

7、carcinomainnudemice2survivinASODNCaninhibittheexpressionlevelofsurvivin，cmycandcyclinD1protein，andmayinhibitthegrowthoftransplantedhumancholangicarcinomainnudemiceKeyword：survivin，cholangicarcinoma，c—myc，cyclinD1，antisen