線粒體外膜易位酶tom40在D-半乳糖誘導(dǎo)的擬老化大鼠內(nèi)耳中的表達(dá).pdf

1、華中科技大學(xué)碩士學(xué)位論文線粒體外膜易位酶tom40在D半乳糖誘導(dǎo)的擬老化大鼠內(nèi)耳中的表達(dá)姓名：郝亞楠申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：耳鼻咽喉頭頸外科學(xué)指導(dǎo)教師：孔維佳20090501華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文ABSTRACTObjective:Toexpletheroleofmitochondrionproteinimptinthemechanismsofagerelatedhearingloss.Method:Onehu

2、ndredtwentyWistarratswereromlydividedintofourgroupseachgrouphadthirtyrats.GroupBCDweretreatedwithhypodermicDgalactose150mgkg1d1300mgkg1d1500mgkg1d1respectivelyfeightweeks.GroupAweretreatedwithsalinefthesamedose.Auditybra

3、instemresponse(ABR)wasusedtodetectthehearingthresholdofratsbefeafterthetreatment.ThemitochondrialDNA4834bpdeletionmutationofinnereartissuewasdetectedbynestedPCRtechniqueindertoevaluatetheestablishmentofthemodel.ThemRNApr

4、oteinleveloftom40weredetectedbyRTPCRtechniquewesternblottechniquerespectively.Results:ThemitochondrialDNA4834bpdeletionwasdetectedinallratsofgroupBCD(303030303030)whileonlyoneofgroupA(130).VariationinABRthresholdofgroupA

5、was(2.503.66)dBpeSPLgroupB(2.833.87)dBpeSPLgroupC(3.503.51)groupD（3.333.30）dBpeSPL.ThedifferenceinshiftofABRthresholdbetweenthefourgroupswasnotsignificant.ThedifferenceofmRNAleveloftom40betweenthefourgroupswasalsonotsign

6、ificant.Thedifferenceofproteinleveloftom40betweengroupABwasnotsignificantbutitwassignificantbetweentheothergroups.TheproteinleveldecreasedalongwiththeincreaseofthedoseofDgalactose.Thecrelationcoefficientwas0.814.Conclusi

7、on:Theproteinlevelsoftom40decreasedinexperimentgroupswhichindicatesthatthetranslocationofnulearencodedmitochondrionproteinsmaydisderalongwiththehappeningofagerelatedhearingloss.Keywds:tom40innerearagingmitochondriondelet