厚皮甜瓜果實(shí)RNA的提取及采后硅酸鈉處理對(duì)其防衛(wèi)基因的誘導(dǎo)表達(dá).pdf

1、甘肅農(nóng)業(yè)大學(xué)碩士學(xué)位論文厚皮甜瓜果實(shí)RNA的提取及采后硅酸鈉處理對(duì)其防衛(wèi)基因的誘導(dǎo)表達(dá)姓名：趙繼榮申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：果樹學(xué)指導(dǎo)教師：畢陽(yáng)20080601甘肅農(nóng)業(yè)大學(xué)2008屆碩士學(xué)位論文Summary—Asprogressingonpostharvestinducedresistanceinmuskmelon，itisnecessarytoanalysistheexpressionofdefensegenesintreatedfr

2、uitInthispaperRNAwasextractedfrommuskmelon“Yindi”(CucumismeloLCVYindi)alterscreeningonmajorfactorsofTrizoImethodTheexpressionofsevendefensegenesWasanalyzedbyRTPCRinfruittreatedwinlsodiumsilicateatlOOmmolandchallengedwi也T

3、richotheciumroseumTheresultsindicatedasbelOW“1ThemostanaountofextractedRNAwasin200ragsamplewimlmlTriz01A260／A280ofRNAwasbeweenl9and20intheeonditionofchloroformextractivedtwiceTheextractedRNAwasnotpollutedbyproteinandDNAa

4、ndWastranscribedreverselyBothroomandlowtemperaturesedimenthadnosinginfiganteffectonRNAyieldAmplifiedgeneflactinWassameasdesignedaftertheproductionofreversetranscriptiondilutedfivetimesItwasprovedthatextractedRNAcouldbeus

5、edforRTPCR2TheeDNAtemplatequantityWashomogeneityafteradjustedseveraltimesThegeneofMCHTPAL，SOD，POD，APXandLOXWasverificatedbystandardMarkermethodCHSWasverificatedwimtestsequenceanalysismethodandthesequenceinmuskmelonWasgot

6、tenTheresultsshowedthatthetargetgenescouldbevertificatedbystandardMarkermethodandthetestsequenceanalysismethodandthedesignedprimerscouldbeusedforexpressionanalysis3ThemelontreatedwithsodiumsilicateandthechallengewithZros

7、eumadvancedMCHTandSODgenesexpressionmaximumfor2days，andtheexpressionoftreatmentWasmorethanthecontr01ThetreatmentandchallengeimprovedPALandCHSgenesexpression，prolongedexpressionmaximumofPODgenesfor2days，andincreasedPODgen

8、eexpressionaftertreatmentandchallengeconditionsHoweverAPXandLOXgenesexpressionwereinhibitedbythetreatmentTheresultssuggestthatfruitsresistanceisinducedbytreatmentofsodiumsilicateKeywords：Silicon；Fruit；RNA；Defensegenes；In