厚皮甜瓜果實RNA的提取及采后硅酸鈉處理對其防衛(wèi)基因的誘導表達.pdf

1、甘肅農業(yè)大學碩士學位論文厚皮甜瓜果實RNA的提取及采后硅酸鈉處理對其防衛(wèi)基因的誘導表達姓名：趙繼榮申請學位級別：碩士專業(yè)：果樹學指導教師：畢陽20080601甘肅農業(yè)大學2008屆碩士學位論文Summary—Asprogressingonpostharvestinducedresistanceinmuskmelon，itisnecessarytoanalysistheexpressionofdefensegenesintreatedfr

2、uitInthispaperRNAwasextractedfrommuskmelon“Yindi”(CucumismeloLCVYindi)alterscreeningonmajorfactorsofTrizoImethodTheexpressionofsevendefensegenesWasanalyzedbyRTPCRinfruittreatedwinlsodiumsilicateatlOOmmolandchallengedwi也T

3、richotheciumroseumTheresultsindicatedasbelOW“1ThemostanaountofextractedRNAwasin200ragsamplewimlmlTriz01A260／A280ofRNAwasbeweenl9and20intheeonditionofchloroformextractivedtwiceTheextractedRNAwasnotpollutedbyproteinandDNAa

4、ndWastranscribedreverselyBothroomandlowtemperaturesedimenthadnosinginfiganteffectonRNAyieldAmplifiedgeneflactinWassameasdesignedaftertheproductionofreversetranscriptiondilutedfivetimesItwasprovedthatextractedRNAcouldbeus

5、edforRTPCR2TheeDNAtemplatequantityWashomogeneityafteradjustedseveraltimesThegeneofMCHTPAL，SOD，POD，APXandLOXWasverificatedbystandardMarkermethodCHSWasverificatedwimtestsequenceanalysismethodandthesequenceinmuskmelonWasgot

6、tenTheresultsshowedthatthetargetgenescouldbevertificatedbystandardMarkermethodandthetestsequenceanalysismethodandthedesignedprimerscouldbeusedforexpressionanalysis3ThemelontreatedwithsodiumsilicateandthechallengewithZros

7、eumadvancedMCHTandSODgenesexpressionmaximumfor2days，andtheexpressionoftreatmentWasmorethanthecontr01ThetreatmentandchallengeimprovedPALandCHSgenesexpression，prolongedexpressionmaximumofPODgenesfor2days，andincreasedPODgen

8、eexpressionaftertreatmentandchallengeconditionsHoweverAPXandLOXgenesexpressionwereinhibitedbythetreatmentTheresultssuggestthatfruitsresistanceisinducedbytreatmentofsodiumsilicateKeywords：Silicon；Fruit；RNA；Defensegenes；In