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1、吉林農(nóng)業(yè)大學(xué)碩士學(xué)位論文黑果腺肋花楸與越橘的原生質(zhì)體分離與融合研究姓名：李昊申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：果樹(shù)學(xué)指導(dǎo)教師：張志東20080601ResearchonProtoplastIsolationandFusionofBlackchokeberryandBlueberryAbstractBlackchokeberry(Aroniamelanocarpa)isanewsmallberryfruitAsBlackchokeberrytobe

2、ofvalueindominantgenerestrictions，makingimprovementsthroughconventionalbreedingmethodscultivarsbegreatlyrestrictedBlackchokeberryrichoffruitanthocyanins，coldhardiness，pestresistancecapabilityBlueberryfruitsofagrowingemph

3、asisonhealthfunction，inadditiontoprevembrainagin舀enhanceheartfunction，antiinflammatoryantiCarlcer，anti—oxidationalsocallbeeffectiveandhavegoodeyesightprotectionUseoftechnicalmeans，blueberryandblackfruitglandribSofousthep

4、rotoplastfusion，itispossibletoovereomethenaturalworldintheformofreproductiveisolationbetweenspecies，therebycreatingallewfineofresourcesThistestuseblackchokeberryandmidbushBlueberryNorthland’leaf邵material，thestudyoftheenz

5、ymeisolatedprotoplastsofcomposition，hydrolysistimeandmethodofsolutionpH，osmoticPressureregulatorconcentration，centrifugalconditions，leafpretreatmentmethods，CPWsolutioncomponentsandinvitroenzymatichydrolysisformermediumof

6、changeonprotoplastPurificationofthebestconditionsinthesetwoplantsandmaterialsforapreliminaryprotoplasttheintegrationofresearch，andadeno—fibbedfruitandblueberrySorbussomaticcellhybridizationpreliminaryexplorationTestmainf

7、indingsareasfollows：1Theblackchokeberryprotoplastsisolatedconditions：25℃41℃，cellulase，pectinase，mannitol07mol／L5mmol／LMES，hydrolysistime810hoursapHvalueof6572Cuttheblackchokeberrytopl一4leafintol2mmlong，enzymatichydrolysi

8、sbetterthanundertheskintornoffleavesgoodHydrolysisthesametime，theuseofstaticandtheroleofdarkhydrolysis30r／mindarkdoubleenzymeoscillationthereWasnosignificantdifference，youcanBSeasimplestaticEnzymaticTherewillbenovaccineh

9、ydrolysisbeforedarkhandleitreducestheoutputofprotoplastsandvitality3Osmoticstrengthoftheoutputrestrictionsprotoplastrolewasclear07mol／Lmannitolunderblackchokeberryprotoplastyieldverydifferentandadjacentprocessing4Theblac

10、kchokeberryhydrolysateofnearlyneutralpHvalue，theenzymeeffectiveInnearlyneutralpHrange，afterenzymehydrolysisofpHvaluessimilarrateofdecline5CPWformulaaddedM92CaneffectivelyimprovethelivingBlueberryprotoplastyield6Withthein

11、creaseincentrifugaltime，Blueberryprotoplastyieldhigher，buttheprotoplastsincreasedactivitydecreasedafterfirst7Blueberryprotoplastsisolatedintheoperationofaddingpectinase，protoplastyieldimprovedto05g|‘Lpectinasebyprotoplas

12、tliveupto334103，gFW8Blueberryvitroenzymatichydrolysisofsucrosecontentinthetrainingbefore20g|Lmedium，thelargestliveprotoplastyieldto46110’／gFW9ObserVedthatasingleprotoplastadhesionprocess，protoplastfusionlinkedtothemembra

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