14-3-3蛋白亞型在人肝癌組織中的表達及意義.pdf

1、南昌大學碩士學位論文1433蛋白亞型在人肝癌組織中的表達及意義姓名：劉桂芬申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：尹東20100601ABSTRACTABSTRACTBackgroundandObjective：1433proteinisagroupofhighlyconservativeacidproteinfamilybydifferentgenesencoding，therearesevensubtypesinmam

2、mals，including，Y，￡，rl，0，0and‘Theexpressionof14—33proteinsinspecifictissuesisisoform—specific14—33proteinscailinteractwithmorethan300targetproteinsinaphosphorylationdependentandphosphorylationindependent14—33proteinsplaya

3、nimportantroleinmanycellularprocesses，suchascellcycle，cellsignaltransductionandapoptosis，andwereshowedacloseassociatewiththecancerdevelopmentandprogressionInthepresentstudytheexpressionof14—3—3isoformsmRNAandproteinswere

4、observedinhumanprimaryhepaticcarcinomaandinhumannormallivertissues，andexploredthecorrelationandsignificancebetween143—3proteinsexpressionlevelandhumanprimarylivercarcinomaMethods：Sixteenhumanprimaryhepaticcarcinomasample

5、sandtentumorfreelivertissuesobtainedfromthehepatectomyThelevelsofallseven1433isoformsmRNAwereexaminedbyRTPCR，andafterstatisticalanalysis，theproteinexpressionwereidentifiedbyWesternBlottingtothoseisoformswhichmRNAexpressi

6、onaredifferentResults：Abasalexpressionofalltheseven1433isoformsmRNAweredetectedbyRTPCRbothinthelivercancertissuesandthenon—canceronesHowevertheexpressionlevelof14—3—3BandrlmRNAweresignificantlyincreasedinhepaticcarcinoma

7、tissuesbycomparingwiththetumorfreetissues(P001)Theproteinexpressionlevelof143313andrlwerealsosignificantlyincreasedinhepaticcarcinomatissuesbycomparingwiththetumorfreetissuesbyWesternBlotting(P001)conclusions：1ThereWasab