電刺激Meynert基底核對血管性癡呆大鼠的學(xué)習(xí)記憶的影響及其機制的探討.pdf

1、電刺激Meynert基底核對血管性癡呆大鼠的學(xué)習(xí)記憶的影響及其機制的探討摘要目的觀察電刺激Meynert基底核(NBM)對血管性癡呆(VaD)大鼠學(xué)習(xí)記憶能力以及海馬CAl區(qū)神經(jīng)元形態(tài)、神經(jīng)生長因子和乙酰膽堿轉(zhuǎn)移酶表達的影響，并初步探討電刺激NBM對VaD大鼠認知功能改善作用及可能的作用機制。方法采用隨機數(shù)字表達法將30只SD大鼠分為空白組、假刺激組及刺激組，選用雙側(cè)頸動脈永久結(jié)扎術(shù)將假刺激組及電刺激組大鼠制成VaD大鼠模型，空白組大鼠

2、僅分離雙側(cè)頸動脈不結(jié)扎。刺激組大鼠于制模后埋入顱內(nèi)電極并給予NBM電刺激，電刺激每次持續(xù)30min，共治療14d。電刺激完成對各組大鼠進行連續(xù)4d的定位航行水迷宮檢測，第5d進行空間探索實驗；完成水迷宮檢測后分別采用尼氏染色和免疫組化染色觀察各組大鼠海馬CAl區(qū)神經(jīng)元變化情況。結(jié)果Moms水迷宮檢測示空白組、刺激組大鼠逃避潛伏期和總游泳距離均較假刺激組明顯縮短(p005)，并且相同時間點均以假手術(shù)逃避潛伏期和游泳距離縮短幅度較顯著?？臻g

3、探索試驗中，刺激組大鼠的平臺象限游泳時間的百分比、通過平臺象限的游泳次數(shù)及平臺區(qū)域游泳距離明顯多于假刺激組，差異有統(tǒng)計學(xué)意義。刺激組尼氏染色結(jié)果與空白組相似，可見神經(jīng)元排列較整齊，存活神經(jīng)元數(shù)量較多，且細胞體積較大，尼氏小體豐富，但數(shù)量不及空白組。免疫組化染色電刺激組[(1987210124)％]NGF陽性細胞百分比及空白組[(209049876)％]陽性細胞百分比均較假刺激組[(66644002)％】明顯增加；刺激組[(1160859

4、84)％]Chat陽性細胞百分比及空白組[(1997510102)％]陽性細胞百分亦較假刺激組[(62773431)％]明顯增加。EffectsofelectricalstimulationofNBMontheratswithvasculardementiaanditsmechanismsAbstractObjectiveToverifythehypothesisthatstimulationofthenucleusbasalisofM

5、eynert(NBM、inratswithvasculardementiacanalleviatethecognitiveimpairment，weinvestigatedwhethertheexpressionofNGFandChatinCA1regionofhippocampalneuronsisincreasedafterstimulationofNBMusingimmunocytochemistrytechniquesMetho

6、dsWeusedadultmaleSprague—Dawleyrats(weight3004009)Ratswererandomizedintoshamoperationgroup，shamstimulationgroupandstimulationgroupVaDratswereestablishedbybilateralvertebralarteryocclusionweperformchronicstimulation(30min

7、perday14days)on2VOratsandexaminethespatiallearningandmemoryinMorriswatermazeThethreegroupperformcontinuous4daysplacenavigationtestandthefifthdayperformspaticalprobeexperimentApplicationNisslstainingandimmunohistochemical

8、stainingtechniquetoobservechangesinhippocampalCA1neuronsofratsindifferencegroupResultsIntheplacenavigationtest，comparedwithmodelgroup，thestimulationgroupwasshorterfollowingelongationofreperfusion(正005)；inthespaticalprobe

9、experiment，Inperformspaticalprobeexperiment，percentageoftimequadrant，thenumberandareabyswimmingswimplatformfromtheplatformquadrantofthestimulationgroupwasthemorethanshamstimulationgroup，thedifferencewasstatisticallysigni