分離自AIDS患者的白念珠菌唑類耐藥株中.pdf

1、新疆醫(yī)科大學(xué)碩士學(xué)位論文分離自AIDS患者的白念珠菌唑類耐藥株中姓名：張麗娟申請學(xué)位級別：碩士專業(yè)：皮膚病與性病學(xué)指導(dǎo)教師：惠艷2010-04新疆醫(yī)科大學(xué)醫(yī)學(xué)碩士學(xué)位論文 新疆醫(yī)科大學(xué)醫(yī)學(xué)碩士學(xué)位論文 2Study on Erg11gene of candida anbicans azole-resistanceisolate from AIDS Postgraduate:ZHANG Li-juan Supervisor: HU

2、I Yan professor Abstract Objective: To investigate the piont mutation of cytochrome P450 lanosterol 14-a demethylase gene Erg11 and its relationship with azole-resistance candida albicans isolated from AIDS. Methods: The

3、 azole-resistance and sensitive isolate (fluconazol, Itraconazole, voriconazole) were selected in vitro according to CLSI M27-A protocols.the DNA was extracted by enzymatic lysis method, the objective gene were divided t

4、wo fragment for PCR reaction respectively, PCR product were sequenced and then contrast with the standarded sensitive strain in Genebank. Results:The 25 piont mutation were found in the 27 azole-resistance strains,and t

5、here are 5 significant site which had made amino acids replacement： D116E、 E266D、 H485T、 V447 and V488I， In azole-resistance strains, all strains happened H485T, five had E266D,three had V488I, one had E266D and V488I si

6、multaneously,and one had V447I, two sensitive strains hanppened significant mutation. Conclusion:In the study,five significant mutation were found which made their coding amino acids replacement,in which H485Tand V447I w