1,25(OH)2D3對肺腺癌細胞增殖分化作用及機制初步研究.pdf

1、上海交通大學博士學位論文1，25（OH)2D3對肺腺癌細胞增殖分化作用及機制初步研究姓名：李榕申請學位級別：博士專業(yè)：內(nèi)科學（呼吸病學）指導教師：韓寶惠201204APRELl川NARYSTUDYOFTHEEFFECTANDMECHANISMOF1，25(OH)2D3ONLUNGADENOCARCINOMACELLPROLIFERAT10NANDDIFFERENTIATl0NAbstractBackground：Theincidence

2、anddevelopmentofmanyhumandiseasesdirectlyrelatedtoimpropernutritionLungcancerisadiseasewithhighincidenceandhighmortalityAdenocarcinomaisthemainpathologicaltypeoflungcancerThetumorstemcellsformrealignantproliferation，tran

3、sfer，highlyresistanttothechemotherapyOurpreviousstudieshaveshownthat：advancedlungcancerpatientSwithmalnutritionareacommonphenomenon；theOCT4BASCiSatypeofCSC1ikecellexistenceinlungcancerandtheocT4tBASCthreattothesurvivalin

4、lungcancerpatientsThecurrentstudyfoundthatvitaminDhasacertainroleintumorprevention，treatment，buttherelationshipbetweenvitaminDandlungadenocarcinomaiSsti】1unclearObjective：MakecleariflungcancerpatientsbewithvitaminDdefici

5、encyAnalyzetherelationshiDbetweenvitaminDlevelSandthepathologicaltypeoflungcancerAnalyzetheroleofvitaminDonthedifferentiationofCSC1ikeAT21ikeandATl1ikelungadenocarcinomacellsAnalyzewhetherl，25(0H)2D3，salinomycin，DRPTCani

6、nhibiteOCT4BASCgrowthandinducedifferentiationof0CT4BASC。andwhetherthereissynergyinthethreeMethods：Chemiluminescencedetectionwasusedtodetectserum25(OH)econcentrationsinthelungcancerpatients。EstablishtheCSC1ike，AT2celIsThe

7、effectofl，25(OH)2D30nonthesecellsweredetectedby1ikeandATI1ikelungadenocarcinomatheexpressionofCCSP，SP—C，OCT4，SP—BimmunofluorescencedetectionTheCCK8assaywasusedtodetecttheeffectof1，25(OH)2D3，salinomycinandDAPTinpr01iferat