人參皂甙對缺血神經(jīng)元的保護(hù)作用研究.pdf

1、蘇州大學(xué)碩士學(xué)位論文人參皂甙對缺血神經(jīng)元的保護(hù)作用研究姓名：李愛紅申請學(xué)位級別：碩士專業(yè)：神經(jīng)病學(xué)指導(dǎo)教師：包仕堯柯開富2002.4.1—S—t—u—d—y—o—f—p—r—o—te—c—ti—x—e—e—ff—e—c—ts——o—fG——i—ns—e—n—o—s—id—e—s—o—n—Is—chemicinjuriesinCulturedmousecorticalNeuronsAbstract——一StudyOfprotectivee

2、ffectsofGinsenosidesonIschemicInjuriesinCulturedmousecorticalNeuronsAbstractobjeetix7e：TostudytheneruoprotectiveeffectsofthreeofGinsenosides((3SRbl、GSRb3、GSR91)onischemicculturedmousecoNicaIneuronsmOnOmerS1njurlesinMetho

3、ds：(DMorphologicalobservationandMTTassaywereusedtocompm’etheeffectsofdifferentdurationofischemiaonculturedmouseconicalneurons②VariousconcentrationsofGSRbl、Rb3、Rgl(20100utool／100m1)wereusedtoobservetheireffectsonjschemicc

4、ultured1／louseconicalneuronsandneuronalviabilitiesinprimaryculturesfr0111nlousecerebralcodexassessedbymeansofMTTassay⑧TheischemicmodelofcerebralcodexneuronculturewasusedTheprotectiveeffectsofGSRbt、Rb3、Rgtonmouseculturedc

5、orticalneuronswereinvestigatedbyrnorphologicalobservation、biochemicalanalysisandneuronalApoptoticrateResults：①TheneuronalviabilitydecreasedOrthecelldeathrateincreasedasischemiadurationprolonged②GSRbl、Rb3、R91couldimprovet

6、heneuronalviabilityrespectivelyinadosedependentrnannerintherangeof20tO60“tool／1001hiTheseeffectsweremostsignificantattheconcentl’ation60Utool／100m1Whileintherangeof60to100utool／1OOml，theprotectiveeffectsofGSdecreasedGSRb

7、l、Rb3、Rglat100tttool／100mlhadnoprotectiveeffects⑧Comparedwithischemiagroup，ThecelldeathratedecreasedsignificantlytogetherwithdecreasinginLDHrelease、Nelease、mDAcontentandcellapoptoticrate，andthesupra‘oxidedismutase(SOD)ac