生脈注射液、bFGF對HIBD鼠神經(jīng)保護作用的研究.pdf

1、東南大學碩士學位論文生脈注射液、bFGF對HIBD鼠神經(jīng)保護作用的研究姓名：張敏申請學位級別：碩士專業(yè)：兒科學指導教師：蔣犁20090531東南大學碩士學位論文AbstractStudyingoftheNeuroprotectiVeeffectofPulse—activatingInjectionandbFGFfortheNeonatalRatsafterHypoxic—ischemicBrainDamageDivisionofPead

2、iatrics，MedicalCollegeofSoutheastUniversityZHANGMin，JIANGLiObjectiveToobservetherelationshipbetweenthehippocampalproliferationanddifferentiationindentategyrusandbasicfibroblastgrowthfaetor(bFGF)andpulseactivatinginjectio

3、nafterhypoxicischemicbraindamage(HIBD)intheneonatalrats；toexplorethelatercapabilityoflearningandmemoryoftheratsMethodsTheneonatalHIBDmodelwasestablishedwith7dayoldratpups，andrandomlydividedintotheshamgroup，thehypoxiaisch

4、emiagroup(HIgroup)，thebFGFgroupandthepulse—activatinginjectiongroup(Pgroup)Ratswerekilledontheday5，10，14and21afteroperation，respectivelyTheslicesofthebrainswereundertakenwitlldoubleimmunofluoresciencestainingtoobservethe

5、expressionofBrdU／MAP2posiitiveorBrdU／GFAP—positivecellsindentategymsYmazeWasusedforthebehaviortestResultsIntheHIgroup，thebFGFgroupandthePgroup，theexpressionofBrdU／MAP2posiitiveandBrdU／GFAPpositivecellsincreasedontheday10

6、and14afterHIBDwhencomparedwithontheday5(pO05)，anddecreasedontheday21InthebFGFgroupandthePgroup，theexpressionofBrdU／MAP2positiveandBrdU／GFAPpositivecellsincreasedontheday5，10，14and21afterHIBDwhencomparedwiththeshamgroupan

7、dtheHIgroup(p005)InthebFGFgroupandthePgroup，improvementsinbehaviorandmemorywereshowedonthehypoxic—ischemiegroup(p005)ConclusionsThereWasneurogenesisindentategyrusofhippocampusafterHIBDandthecelBcouldproliferateanddiffere

8、ntiateintothenewneuronsandastrocytesThetherapyofexogenousbFGForpulse—activatinginjectionpromotedcellproliferationanddifferentiationindentategymspossiblyandimprovedthecapabilityoflearningandmemoryoftheratsItcouldbeconclud

9、edthatexogenousbFGFandpulse—activatinginjectiontakepartinthemechanismofendogenousneurogenesisafterHIBDpossiblyKeywords：Cerebralhypoxia；Cerebralischemia；dentategyriK$；differentiation；BasicFibroblastGrowthFactor：pulse—acti