EB病毒相關(guān)胃癌細(xì)胞凋亡與bcl-2表達(dá)的研究.pdf

1、青島大學(xué)碩士學(xué)位論文EB病毒相關(guān)胃癌細(xì)胞凋亡與bcl2表達(dá)的研究姓名：姜曉華申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：病原生物學(xué)指導(dǎo)教師：羅兵20030910英文摘要一—、————————————_ww_m—H_———————H_———wwmⅢⅢ__h_—“—’———————__HⅢw_——●—————————_H—m_mwm●———————H__—HmmⅢmⅧw●———————————w__～APOPTOSISANDEXPRESSIONOFbcl一2I

2、NEP～STEIN一8ARRV王RUSASSoC王ATEDGASTRlCCARCINOMAABS鬻裂ACTObjectiveEBVhasbeenfoundinmostcasesofraregastriclymphoepithelioma—likecarcinomaandasmallbutsignificantproportionofcommongastricadenocareinomaEBVDNAcouldbedetectedinabO

3、ut7～10％gastricadenocarclnomasHowever，thepathogeneticroleofEBVingastriccarcinomaisuncertainInordertoexplorethepotentialpatho—genieroleofEBVencodedproteinsandbcl一2proteinintheoncogenicpathway，wedetee—tedtheexpressionofsome

4、EBV—encodedproteinsinEBV—associatedgastriccarcinoma(琶BVaGe)，comparedtherateofapoptoticcellsandtheexpressionofbcl2proteininE&VaGCandclinieopathologicaldatamatchedEBVnegativegastriccarcinoma(EBVnGC)MethodsTheapoptoticindex

5、(AI)andtheexpressionofbcl一2proteinweretestedbyterminaldeoxynucleotdyltransferase(TdT)一mediateddUTP—biotinnickendlabelling(蕈UNEL)andimmunohistochemistryrespectivelyin13E轉(zhuǎn)VaGesand45EBVnGCsTheexpressionOfrelatedgenesofEBVwa

6、stestedbyRT—PCRandSouthernblottingResults①AIofEBVaGCs，EBVnGCsandthecorrespondingadjacenttissuesofEBnVaGCswere0969204131，20333士0，6000and3。25380。4594respectively。AIof囂BVaGCwassignificantlylowerthanthatofEBVnGC(￡=9795，P一0)a

7、ndcorrespondingadjacenttissuesofEBVaGC(t=132229，P一0)②Thenumberofapoptoticcellswassignificantlylowerinbel一2一postive(15793士06946)turnoutsthanbcl’2一negativetumours(2，0103圭06842，P0。05)③Theexpressionofbcb2proteinxⅣasdeteetded

8、in29of58(5000懿)gastriccarcinomasandin17(2931％)of58adjacenttissuesThereWaSsignif～leantdifferencebetweenthetWOgroups(％2—43214，P=O0376)Theexpressionofbcl2proteinwasdetectdedin7of13(5385％)EBVaGCsandin22of45(4889％)EB～VnGCs。Th

9、ediHerencebetweenthetWOgroupswasnotsignificant(釅一00991，P207529)④EBNAlmRNAwasdetectedinallof13EBVaGCs，whilebothEBNA2andLMPlmRNAwerenotdetectedin13casesOfthe13EBVpositivesamples，6exhibitedBARFltranscriptsand2exhibitedBHRFl

10、transcriptsConclusions④hcl一2expressiondidnotcorrelatewiththepresenceofEBVinEBVaGC②SignificantlowrateofapoptosisinEBVaGCsuggestedthatEBVinfectioncouldinhibitcellapoptosisHowever，themechanismbywhichEBVinhibitcellapoptosisd