CIK細(xì)胞在血液系統(tǒng)惡性腫瘤的應(yīng)用研究.pdf

1、東南大學(xué)碩士學(xué)位論文CIK細(xì)胞在血液系統(tǒng)惡性腫瘤的應(yīng)用研究姓名：孫耘玉申請學(xué)位級別：碩士專業(yè)：內(nèi)科學(xué)指導(dǎo)教師：陳寶安20060320東南大學(xué)碩士學(xué)位論文AbstractT砒e：ApplicationofCIKtomalignanthematologicaldiseaseCandidateMaster：SUNYunyuSupervisor：ProLCHENBaoanNameoftheUniversity：SoutheastUniversi

2、tyobjective：ToobserveandevaluatethesafetyandtherapeuticeffectsofcytokineinducedkillercellsOUthepatientswithhematologicaltumorMethods：SeparatebloodcellstomakePBMC(peripheralbloodmononuclearcell)withFieolls，thenculturedint

3、hemediumcontaininginterferonr(IFNr)interleukine2(IL2)andantibodyagainstCD3(antiCD3)forabout14to21days11”phenotypeofCIKcellswereidentifiedbyflowcytometricanalysisCytotoxicactivityusiIlgMTTassayCytokineinducedkillercellswe

4、reinfusedbacktothepatientsafterculturedfor15—2ldaystoobservethechangeoftheTcellsubsetsandNKcellsinperipheralblood，toevaluatetheimprovementofthedinicMsyndromesandthesideeffectsResults：cIKcellscanbeexpandedeffectivelyfromt

5、hehealth多donorsandthepatientswithhematologicaltumorandmediateexvivocytotoxicityagainsthematologicalmalignancieswithMTTtestFivepatientsweretreatedwithCIKcellsinfusionItwasobservedtheimprovementclinicallytheTcellsubsetsand

6、NKcellschangedafterandbeforetreatmentConclusion：CIKcellscanbeexpandedeffectivelyfromhealthydonorsandthosepatients誦tlladvancedhematologicalmalignancies；theyCallmediatecytotoxicityagainsthematologicalmalignancies：Itisaprom

7、isingformoftheimmunothempyinthehematologicalmalignanciesItcarlimprovetheimmuneabilityreliefthedinicalsyndromeimprovethequalityofpatient’Slife，prolongtheperiodofsurvivalKeywords：hematologicalmalignanciesimmunotherapycytok