哈、漢民族食管癌抑癌基因DNA異常甲基化及其與預(yù)后關(guān)系的研究.pdf

1、新疆醫(yī)科大學(xué)碩士學(xué)位論文哈、漢民族食管癌抑癌基因DNA異常甲基化及其與預(yù)后關(guān)系的研究姓名：趙燕申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：衛(wèi)生毒理學(xué)指導(dǎo)教師：陳艷201104新疆醫(yī)科大學(xué)碩士學(xué)位論文新疆醫(yī)科大學(xué)碩士學(xué)位論文2StudyonDNAhypermethylationofesophagealtumsuppressgeneinKazakHannationalitiesitsrelationshipwithPrognosisPostgraduate:Z

2、haoYanSupervis:Prof.ChenYanAbstractObjective:Toexpletherelationshipbetweenthehypermethylationstatusofp16FHITgenesinHazakHanpatientswithesophagealcarcinomathedevelopmentofesophagealcanceranalysetheprognosis.Methods:Thehyp

3、ermethylationofthep16FHITgenesweredetectedbymethylationspecificpolymerasechainreaction(MSP)in71casesofHazakHanpatients’esophagealcancertissue.thentoanalysetherelationshipamongthemethylationstatusagegenderlymphnodemetasta

4、sisDifferentiationclinicalstagingtheprognosis.Results:In36casesofHazakpatientswithesophagealcancerthep16genemethylationinesophagealsquamouscellcarcinomatissuesamplesadjacenttissuesampleswere44.4%13.9%theFHITgenemethylati

5、onwere58.3%19.4%.In35casesofHanpatientsthep16genemethylationinesophagealsquamouscellcarcinomatissuesamplesadjacenttissuesampleswere42.9%14.3%theFHITgenemethylationwere51.4%14.3%.Thedifferenceofp16genemethylationbetweenka

6、zakHanpatientswasstatisticallysignificant(χ2=7.360P=0.007χ2=7.000P=0.008)thedifferenceofFHITgenemethylationwasstatisticallysignificanttoo()(χ2=11.455P=0.001χ2=10.944P=0.001).Thedifferencebetweenkazakhannationalitiesinp16

7、genemethylationwasnotstatisticallysignificant(χ2=0.018P=0.893χ2=0.002P=0.962)thedifferenceofFHITgenemethylationwasnotstatisticallysignificanttoo()(χ2=0.342P=0.559χ2=0.336P=0.562).MultivariatePrognosticanalysisshowedthatt

8、hedepthoftuminvasionpathologicalstagelymphnodemetastasisofthenumbersofesophagealcancerareawereindependentprognosticimpactofriskfactsgenderwasaprotectivefactfprognosisofesophagealcancer.Conclusions:Thep16FHITgenesmethylat