花青素調(diào)節(jié)基因Lc與黑色素基因?qū)D(zhuǎn)基因煙草和矮牽?；ㄉ挠绊?pdf

1、Y7743分類號：密級：飛嚼差≮犬等碩士學(xué)位論文單位代碼；10019學(xué)號：S02418』“花青素調(diào)節(jié)基因￡c與黑色素基因?qū)D(zhuǎn)基因煙草和矮牽?；ㄉ挠绊慣heInfluenceofamaizereguolatorgene￡candamelaningeneonflowercolouroftransgenicpetuniasandtobaccos研究生：楊翅春指導(dǎo)教師：朱登云副教授申請學(xué)位門類級別z理學(xué)碩士專業(yè)名稱：遺傳學(xué)研究方向：植物基因工

2、程所在學(xué)院：生物學(xué)院二零零五年六月AbstractThe州mersweredesignedaccordingtothesequenceofBrassicanapns’(rapes’)petalspecificpromoter(GeneBankNoBD082011)，andthepromoterwasamplifiedbyPCRwhichWasnamedXY355TheXY355promoterWasconnectedwithmaize’

3、SanthocyaninregulatorygeneLcandwithamodifiedmelaningenerespectivelytoconstructexpressionvectorspXY60andpYC26TheexpressionvectorspYC26，pXV60，andpQT56(theexpressionvectorprovidedbyourlaboratorywascontainingCaMV35promoteran

4、d厶gene)wereintroducedtotheAgrobacteriumtumefaciencesLBA4404ThentheleafdiscsofpetuniasandtobaccosweretransformedbyAgrobacteriumtumefaciencesLBA4404SomeregeneratedplantshadbeenchosedatrandomandcheckedbyPCRAmongthesepaints，

5、therewere61positivetobaccosincluding23plantsofpYC26，20plantsofpXY60，and18plantsofpQT56，andtherewere31positivepetuniasincluding15plantsofpYC26and16plantsofpXY60Transgenicplants’fiowersCOlorhadbeenobservedThenegativecontro

6、lpetunialines(transformedbypBll21)hadpurpleflowertubesandwhitecorollas8plantsof16pXY60PCRpositivepetuniashadiightpurplecorollasMostpYC26positivepetuniashadnotevidentalterationofflowercolorHoweveroneofpYC26PCRpositivepetu

7、niashadchangeditsflowercolorcausedbaaltermlonoftheflowershapenlenegativecontrolpetunialinesrtransformedbypBIl21)hadpinkpetals10plantsofme20pXY60PCRpositivetobaccoshadredpetalsandantherfilaments15plantsof18pQT56PCRpositiv