阿里紅多糖液體發(fā)酵及提取工藝的研究

1、石河子大學碩士學位論文阿里紅多糖液體發(fā)酵及提取工藝的研究姓名：張濤申請學位級別：碩士專業(yè)：農(nóng)產(chǎn)品加工及貯藏工程指導教師：婁愷20090601AbstractTheprocessofliquidfermentationbyFomesoffcinalisAnlesofUyghurTraditionalMedicaltoproducepolysaccharidewereinvestigatedunderlaboratoryconditions

2、Themainresearchcontentsandresultsareasfollows：(1)WildstrainsofFomesoffcinalisAaTIeswereseparated，purifiedandpreservedusingthesolidplateandslantofrichPDAAtthesametime，theculturetemperatureandtimewereinvestigatedpreliminar

3、ilyTheresultsshowedthatFomesoffcinalisAITIeSCangrownormallyatthetemperaturerangeof15—30℃andgrowthtendtostopaftercultivated8—10days(2)Inordertoobtainthesuitablenutritionconditionsofseedmedia，singlefactorandorthogonaltestw

4、erecarriedoutforthebestchoicesofsortandconcentrationofcarbonsource，nitrogensource，metallicionandgrowthfactorTheoptimalcontentofseedmediaobtainedaccordingtotheyieldofbiomasswereasfollows：glucose3％，peptone15％，KH2P04O3％，MgS

5、047H2001％，VB101mg／mLTheresultsshowedthattheyieldofdrymasscouldreachamount12mg／mLwiththeoptimizedseedmediaandcultivatedl2d(3)Fermentationmediawaschoseandcomponentsoffermentationwereoptimizedbysinglefactorandorthogonaltest

6、Theresultsshowedthat，cultivated12d，theyieldofdrymassandextracellularpolysaccharidecouldreachamount131mgj7mLand15mg／mLrespectivelywiththeoptimizedfermentationmediathatglucose1％maltextract03％，peptoneO75％，yeastextractpowder

7、O5％(4)ThefermentationconditionsofinitialpH，brothcontent，shakingspeedandcultivatemethodetcwerefurtherexploredonthebasisofoptimizationresultsofmediumbysinglefactorandorthogonaltestTheresultsshowedthatundertheoptimalculture

8、conditionsofYMmedium，initialpH50，brothcontent75mL／500mLflask，20。C，shakingspeed180r／minandbatchcultureindarkplace，amount136mg／mLofmyceliabiomassand160mg／mLextracellularpolysaccharideyieldcouldbeobtainedrespectivelyafter12

9、daysinsubmergedcultures(5)ForconfirmthebestextractiontechnologyofpolysaccharidesofFomesoffcinalisAmesastheaimproductionoffermentation，wecontrastivelystudiedtheextractionofhotwaterdiluteacid，dilutealkaliandultrasonicandas

10、certainedfinallythatthebestextractionprocesswasultrasonicassistedhotwaterextractionInordertoobtainingthesuitableextractionprocess，singlefactorandorthogonaltestwerecarriedoutandrecoveryratewastheonlytargetTheresultsshowed