23993.天然產(chǎn)物中抗氧化活性成分的高速逆流色譜分離研究

1、Candidate：f1●一’一SuperVlsOr：Yuan|iangPan_■●●■■■■■■●■■■■●●■●■■■■■■●■_____■■■一DepartmentofChemistryYuquanCampus，ZhejiangUniversityHangzhou，PRChma，310027Jan2014ABST鼬忙TABSTRACTSeparationandpurificationofbioactivenaturalproduc

2、tsisimportantinorganicanalyticalchemistryanddrugdiscoveryAsaliquid—liquidchromatographyCountercurrentchromatography(CCC)haswidelybeenusedintheseparationofnaturalproductsTherefore，methodologystudyforseparationofbioactiven

3、aturalproductsviacountercurrentchromatographyisnotonlymeaningfulinsciencebutalsovaluableineconomyThespecificresearchworkswerelistedasfollows：Inthefirstpart，thebioassayguidedseparationofantioxidantsfromAmpelopsisheterophy

4、llaWascarriedoutviaCCCTheantioxidantactivityofthecrudeextractwasinitiallyevaluatedbyonlineHPLCmethodFivecompoundsinthecrudeextract，namelyhyperoside(1)，isoquercitrin(2)，rutin(3)，kaempferol3rutinoside(4)andquercetin(5)，exh

5、ibitedgoodantioxidantactivitiesThesecompoundswerefurtherseparatedbycountercurrentchromatographyusingbiphasicsolventsystemsTheirstructureswerethenidentifiedbymassspectrometryandNMRAllthecompoundsexhibitedsignificantDPPHra

6、dicalscavengingactivities、析廿ltheICsovaluesat18213，17014，24212，38117and9012∥M，respectivelyThescavengingratiosofthecompoundsagainsthydroxylradicalswere655％，684％，962％，704％and982％，respectivelyThisstrategycouldimprovethesepar

7、ationefficiencyandmayhavepromisingapplication111thesecondpart，twotargetbioactivecompounds，namelyapigenin一7Oglucosideandacacia7Oglucoside，wereseparatedfromChrysanthemummorifoliumviacountercurrentchromatographywiththesolve

8、ntsystemMtBE／nButanol／ACN／H20(15／2／1／5，v／v)Moreovertherecentprogressofcountercurrentchromatography，includingtheelutionmodes，isalseviewedinthispaperKeywords：CountercurrentChromatography；Antioxidant；Ampelopsisheterophylla；