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1、浙江大學(xué)碩士學(xué)位論文石刁柏雄株離體再生體系的建立及生理生化研究姓名：馮莉申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：蔬菜學(xué)指導(dǎo)教師：壽森炎2003.5.1AbstractAsystemstudyofasparagus叫sparagusofficinalisL。)tissueculturewascarriedoutinthispaper，Shootsinclusterwereproliferatedfromsubculturingofmaleasparagu

2、saxilarybudsThenthestemwithonelateralbudwasusedasexplantstostudythemainfactorstothemultiplicationandrootingofasparagusinvitroResultsshowedthatbothKTand6BAacceleratedtheproliferationofshootsclusterTheformertookbeneficiale

3、ffectontherateofmultiplicationandthequantityoftheadventitiousshoots7Fhelatermadetheshootschunky5lhevirtuewasboostedupwheneitheroftheywasaccompaniedwithNAAThekindsandconcentrationsofexohormoneswerethekeyfactorsthataffectr

4、ootinginvitroTheinducingmediumwith10mg／LPP333wassuccessfulinrootingstemcuttingswiththehighestrateof8158％Besides，ShootsnumberoftheregenerateplantswasalsothebiggestinthistreatmentRootingeffectsof0。5mg／L$3307wasassimilarast

5、heformer)therootingratewasabout80％，withvigorousrootsystemsandregenerateplantsRootingrateofthetreatmentwith1Omg／LIBAwere70％approximatelyRootingpercentageofBRtreatmentvaluedfromthelowerconcentrationAbouthalfofplantletsroot

6、ed，whereasplentyofcalliwereproduced，whichdidharmtootingandrootqualityTheresultofthestudybyorthogonaldesignshowedthattheopticalconditionforexplantsrootingwastheinducingculturemediumsupplementingwithPP3331Omg／LIBA2，0mg／LNA

7、A10mg／LIAA05mg／LKT005mg／LTheresultsalsoindicatedthat1／2MSmediumwasmuchbetterforrootinductionthanMSmedium79dayswasasuitableinducingperiodtoinitializetheadventitiousrootsinvitroTheapexofshootwaseasiertoberootedthanthemidse

8、ction。Therootrateoftheshootssubculturedforonetimewashigherthanthatoftheshootssubculturedforthreetimes，ThestemsofasparaguswereculturedtostudythelawsofbiochemicalcharacteristicsduringthecourseofplantregenerationTherewerech

9、angesofdifferentdegree，howeversimilartrendintheactivitiesofPOD，NSEandPALAijthepeaksoftheactivitiesofthesethreeenzymecorrespondedwiththemorphologicalcharactersduringthecourseTherewerealsoformedsomespecificbandsofPODandNSE

10、inthedifferentstagesofplantregenerationThroughanalysisthechangeofsolubleprotein，DNA，RNAandendogenouspolyamines，wefoundthatthecontentsofthemweredeceasedandincreasedsignificantlyduringtheregenerationofplant新vitroItseemstha

11、tailofthesechangesmighthavecloserelationshipwithmorphosisespeciallytheadventitiousrootsandvegetativebudsgrowingApreliminarystudyonanatomyofformationofadventitiousrootsofasparagusinvitrowasalsoconductedTheoriginpositionof

12、therootwasdiscussed。PrecedingconclusionsishelpfultotheresearchofphysicalmechanismofadventitiousrootjnasparagustissuecultureKeyWords：Asparagus秘sparagusofqcimd扭乙，)；all—maleplants；tissueculture；adventitiousroot；physiologica

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