擬南芥GA2oxl基因超量表達對矮牽牛株型的影響及其莖特異表達載體構建.pdf

1、西南大學碩士學位論文擬南芥GA2oxl基因超量表達對矮牽牛株型的影響及其莖特異表達載體構建姓名：葛文東申請學位級別：碩士專業(yè)：花卉學指導教師：郭余龍；李名揚20120603ImpactsofoverexpressionofG彳20％J仔om彳，，祝6f卻s婦onplantt、巾ei11pPf甜，zf口s，andconstn】ctlonotastemspecl士lc^’，promoter—ihVen彳fG么20％JexpressionVe

2、torF10ricul劬舊MaSter黟aduatestudent：Gew％dongSupervisor：AssociatePr0￡YlllongGuoPr0￡Ming)，angLiAbstract11hehei曲tcon仃0lofomamelltalp1鯽tcanberealizedtllepu叩oseofincreasingom鋤踟talqul塒，controllingmeplanthei曲t，fost萌ngtllenewtypeo

3、fdwarf酣nowers，will∥eatlycxp柚dmeflowerinduS_ⅡydeVelopmentSpaCeTherearemanygellestllatregulatetllepl鋤thei曲t，manyoftll鋤a舵ct百bberellicacidsyntllesisGibberellicacidis也emosth印onarltgrowlllho肌onetIlatc鋤C0n廿0l廿lep1觚theight，GA2ox

4、id嬲eisnlekeyenZyme廿latenC0ded西bberellicaCidsynthesis，ca協(xié)11rzcdtllebioactiveGAch卸gefortllea甜啊tyofGAGA2oxid舔eov凹eXpressioIl’wouldreducemeleVelof肌dogellousGA，s0雒toachieVe也epurposeofmal(ingp1鋤tdw橢GA2oxidaSeg朗enot砌ypromote也es

5、t鉍elongationaIldgr0、vm，alsoinfluenCeflowdng鋤d丘1lctificationThetaI苫烈geneexpressediIl也eplantsuccess觚caIIreducepl鋤thei曲t，鯽dmal(eflow萌ng觚d觚嘶ficationabnor鋤a1Ch00singspecificpromoterisabetterChoice111thisexp砸mentwec10nedarabid

6、opsisstemspeci矗callyexpressedpromot％0b協(xié)in胡tllearabidopsisGA2oxidaseg鋤e，cons仉lctedGA2oxidaSegeneoverexpressionvector鯽dspec灑caleXpressionpromot既Weintroducedmeg％eintopetlmi鵲viaagrobact甜ummediatedtechnique，obtaineddwarf甜仃ans

7、gellicplantsUsings】pedficpromoterregulateGA2oxidasegeneexpressedinplantspecificorg鋤，mal(ing舀bberellicacidleVelchangesres們ctedinthestem，oVercomingtheprobl鋤soffloweringand丘Ⅵctificationbecauseof舀bberellicacid’spleionopic，fo

8、stering也enewt、，peofdwarf酣flowersht11isstudyweclonednlegene0fAtGA20x1，constnlctedmeAtGA20xloverexpressedc明ier；Is01atedthepromotersequenCeof鋤dAt3956700gcne，cons仃uctedmespecific“eXpressionvectorofAtGA20x1gelleIn仃(ducedthege