蘇云金芽胞桿菌殺線蟲晶體蛋白在擬南芥中的表達(dá).pdf

1、南開大學(xué)碩士學(xué)位論文蘇云金芽胞桿菌殺線蟲晶體蛋白在擬南芥中的表達(dá)姓名：賈晶月申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：蔡峻201205Abstract——_———————————————————————————————●——————_———————————————————————————————一———————————————————————————————一AbstractPlant。parasiticnematodes(PPNs)a

2、remajoryieldlimitingfactorsinmanycropsThedevelopmentofBacillusthuringiensis(Bt)transgenicinsectresistantplantsisaeffectivewaytocontrolPPNsTheuseofArabidopsisasahostforplant—parasiticnematodeswillprovideanewmodelsystemfor

3、themolecularnematodeintoxicatedmechanismOurlaboratoryhaspreviouslyobtainedaBtnematicidalcrystalproteingenecry6Aa2(GenBankNumberDQ257287)Itsproductintoxicatesfree1ivingCaenorhabditiselegansWehavealreadygotanenhancednemati

4、cidalmutantbysitedirectedmutagenesisInordertoexpressthecry6Aa2mutantinArabidopsisthaliana，thegenewassynthesizedandmodifiedbyremovingsomedestabilizingsequencesandcodonsnotcommonbasedontheArabidopsisgenomepriortointroducei

5、ntoAthalianaviaAgrobaceriumrhizogenesTheCry6AexpressingplantsselectedbyPCRandWesternblottingcoulddecreasethenumberofgallscausedbyroot—knotnematodeMeoidogyneincognitaOurstudyisthefirstreportofexpressingtheBtnematicidalcry

6、stalproteininArabidopsisthalianaTheresultswillhelpUStocontrolplantplant—parasiticnematodesandcreatetransgenicplantsaswellasdeepenourunderstandingoftheinteractionbetweenplant—parasiticnematodesandtransgenicplantsKeywords：