黃鰭鯛D－loop基因序列多態(tài)性及幾種鯛科魚類的分子系統(tǒng)學(xué).pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文黃鰭鯛D－loop基因序列多態(tài)性及幾種鯛科魚類的分子系統(tǒng)學(xué)姓名：劉紅艷申請學(xué)位級別：碩士專業(yè)：水產(chǎn)養(yǎng)殖指導(dǎo)教師：龔世園江世貴20030501PolymorphismoftheMitochondrialDNADloopGeneSequencesfromSparuslatusandMolecularPhylogenyofSomeSpeciesofSparidaeAbsract580bpmitochondriMD—lo

2、opgenesegmemsofSmruslatuscatchedinseafromHaikou(Hainanprovince)，Xiamen(Fujianprovince)，Zhuhai(Guangdongprovince)wereamplified、ⅣitlPCRtechniqueandsequencedHighgeneticpolymorphisminthreepopulationswasexamined33nucleotidesi

3、tes(57％)werevariable22haplotypeswerefoundin24individualInmolecularphylogenetictreeconstructedbYUPGMAmethod，allthehaplotypesofHainanpopulationassembleonebranch，whilethehaplotypesofFujianandGuangdongpopulationsintermixtoge

4、therandassembleanotherbranchNucleotidediversityofsequencesdemonstratesthatHainanpopulationhasafargeneticrelationship晰t11FujianpopulationandOuangdongpopulation，andthatFujianpopulationhasacolserelationshipwithGuangdongpopu

5、lation408b口mitochondrialcytochromebDNAofSpareslatusSmacrocephalusPagrosomusmajorandRhabdosargussarbaweresequencedCombined、vitlltheDNAfragmemsofsberdBTaiustumifrol怙EwnnisjaponicaandArgyropsspm塘rinGeneBank，sequenceswereali

6、gnedandanalyzedThemare126nucleotidesitessubstituted(307／o)SmacrocephatusandSberdaarethemostrelatedofallSparidaefisheswhileftumifronsandRsarbaare也eleastrelatedMolecularphylogenetidtreeisconstructedbyNJandMPmethodofMAGE20T

7、heclassedmsultofRhabdosargus，TaiusandSparusisinconsistentwi也thatofmorphologywhile戶majorEjaponicaandAspm／#rassembleonebranchinNJmolecularphylogenetidtree，PmayorandEjaponicaassembleonebranchinMPmolecularphylogenetidtxee，wh