骨髓增生異常綜合征論文：mds、aa和al患者骨髓細胞周期及增殖特征的研究

1、骨髓增生異常綜合征論文： 骨髓增生異常綜合征論文：MDS MDS、AA AA 和 AL AL 患者骨髓細胞周 患者骨髓細胞周期及增殖特征的研究 期及增殖特征的研究【中文摘要】檢測骨髓增生異常綜合征(myelodysplastic syndrome, MDS)、再生障礙性貧血(aplastic anemia, AA)和急性白血病(acute leukemia, AL)患者骨髓單個核細胞(bone marrow mononuclear c

2、ells, BMMNC)的細胞周期分布及 CD34、Ki67 抗原的表達。方法選取 2009 年 6 月至 2010 年 6 月在山東大學(xué)附屬省立醫(yī)院就診的住院及門診患者 68 例,MDS 組 30 例,其中難治性貧血(refractory anemia, RA)組 18 例,難治性貧血伴原始細胞增多(refractory anemia with excess of blasts, RAEB)組 12 例；AA 組22 例,其中急性重

3、型再障組 7 例,慢性非重型再障組 15 例；AL 組 16例,其中急性髓系白血病(acute myeloid leukemia, AML)組 12 例,急性淋巴細胞白血病(acute lymphoblastic leukemia, ALL)組 4 例。另外選取正常對照組 18 例(均為非血液病患者)。采用常規(guī)骨髓穿刺術(shù),抽取骨髓各 2ml；應(yīng)用流式細胞儀(flow cytometry, FCM),通過碘化丙啶(propidium io

4、dide, PI)細胞核染色及免疫熒光雙標(biāo)法,對其 BMMNC 的細胞周期分布及 CD34、Ki67 的表達進行檢測。結(jié)果1.MDS 和 AL 患者與正常對照組患者骨髓單個核細胞中細胞周期分布及增殖特征表達的比較與正常對照組相比,MDS 組和 AL 組患者其BMMNC 中細胞周期 G0/G1 期、造血干/祖細胞(haemopoietic stem cell, HSC)CD34 細胞、Ki67+細胞、CD34+Ki67+細胞、CD34+細

5、胞中和 AL 患者。4.RA 和 AA 患者相比,其骨髓細胞周期分布及增殖特性的表達差異均具有顯著性意義,可用于二者的診斷及鑒別診斷,尤其是 BMMNC 中 CD34+Ki67+細胞比例兩者差異更大,用作本研究 RA 與 AA的鑒別診斷指標(biāo)準(zhǔn)確率達 100%。【英文摘要】To explore the cell cycle distributions and the expressions of CD34 and Ki67, the ce

6、ll proliferation-related protein in bone marrow mononuclear cells among myelodysplastic syndromes, aplastic anemia, and acute leukemia patients.METHODS Bone marrow aspirates were collected from 68 patients between

7、June 2009 and June 2010 from the Department of Hematology, Provincial Hospital affiliated to Shandong University. The 68 cases consist of 30 MDS, including 18 refractory anemia and 12 refractory anemia with excess blasts

8、,22 AA, including 7 severe aplastic anemia and 15 none severe aplastic anemia, and 18 AL, including 12 acute myeloid leukemia and 4 acute lymphoblastic leukemia.18 healthy individuals (non-hematologic patients) were used

9、 as normal control. Take 2 ml bone marrow from the patients. Propidium iodide and immunofluorescent double staining through flow cytometry were utilized to explore cell cycle distributions and the expression of