金屬還原酶FreB2對煙曲霉鐵吸收及氧化壓力應(yīng)答的影響.pdf

1、A 分類號：R373 密級：不保密UDC： UDC：578 學(xué)校代碼：11065碩士學(xué)位論文金屬還原酶 FreB2 對煙曲霉鐵吸收及氧化壓力應(yīng)答的影響 金屬還原酶 FreB2 對煙曲霉鐵吸收及氧化壓力應(yīng)答的影響王偉偉指 導(dǎo) 教 師 吳翠嬌 教授學(xué) 科 專 業(yè) 名 稱 細胞生物學(xué)論 文 答 辯 日 期 2 0 1 6 - 0 5 - 2 8The Effects of Metalloreductase FreB2 in the Uptak

2、e of Iron andResponse to Oxidative Stress in Asperillus fumigatusAbstractObjective Aspergillus fumigatus is an important opportunitic fungal pathogen，butits pathogenic mechanism is not known. To explore the function of t

3、he gene ofmetalloreductase AFUA- 1G00350 ,termed FreB2, and the role of this gene in theuptake of iron in Aspergillus fumigatus.Method he activity of ferrireductase was compared between the wild type strain andthe mutant

4、 strain in the AMM and -FeAMM, respectively, curves of ferrireductaseactivity of the wild type strain and the mutant strain in different time and growthconditions were drawn. Using the method of Real - Time PCR ， the mRN

5、Aexpression levels of SreA, SidA, FetC, FtrA and FreB which are important genes inthe uptake of iron in Aspergillus fumigatus were analyzed. The sensitivity of the wildtype strain and the mutant strain to oxidative stres

6、s were determined. Then thegeneration of reactive oxygen species were evaluated.Results The ferrireductase activities of the mutant strain were higher than the wildtype strain in both AMM and -FeAMM. Compared with the wi

7、ld type strain, theexpressions of SreA, SidA, FetC, FtrA and FreB of the mutant strain were muchhigher. The deletion of FreB2 results in an increased sensitivity to H202 and thegeneration of reactive oxygen species incre

8、ased remarkably.Conclusion The gene of FreB2 plays a role in both the uptake of iron and oxidativestress response in Aspergillus fumigatus. The genes of the uptake of iron inAspergillus fumigatus have a functional comple