魚(yú)類(lèi)IL-15及其受體α鏈基因家族的分子克隆、進(jìn)化和功能研究.pdf

1、浙江大學(xué)生命科學(xué)學(xué)院博士學(xué)位論文魚(yú)類(lèi)IL15及其受體α鏈基因家族的分子克隆、進(jìn)化和功能研究姓名：方瑋申請(qǐng)學(xué)位級(jí)別：博士專(zhuān)業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：邵健忠項(xiàng)黎新20090430浙江人學(xué)博士學(xué)位論文摘要AbstractChapter1ofthisdissertationisanintroductionoftherecentresearchesonIL15anditsspecificreceptorIL一15RaItwasdescribedin

2、threepartsInthefirstpart，wemainlyintroduceinterleukin一15(IL15)includingitsdiscoverymolecularcloninggenestructure，regulatorymechanismsofitsexpression，aswellasitsreceptorsandthecorrespondingsignalpathways；andthesecondparti

3、ntroducesthemolecularcloningandcharacterizationofIL一15Ra(IL一15receptoralphachain)gene，includingitsexpressionandvarioustypesofalternativesplicingIL15RaspecificallyrecognizesIL15inanunusuallyhi曲affinitywhichcouldaccountfor

4、itsCO—secretion謝mIL15，thevariouseffectsofsolubleIL15Ra，andtrans—presentationofIL15signalInthethirdpart，weintroducethebiologicalfunctionsofIL一15andIL15Raondifferenttypesofimmune—relatedcells，includingNaturalKillerTCRaIBTl

5、ymphocytesandSOon，aswellasIL一15／IL15RaeffectsinthepathologiesofsomeimmunediseasesChapters25arethedescriptionofthemainresearchesInChapter2，weperformedthemolecularcloningandcharacterizationoftheIL15’SorthologuefromTetraodo

6、nnigroviridis，ateleostspecies，aswellasitsprokaryoticexpressioninEcoliandthepurificationofsolublerecombinantproteinBycomparativegenomicsandbioinformaticsmethods，wecarriedoutthestudyontheevolutionofvertebratesIL15genes，inc

7、ludingafishspecificIL15Xgene，whichthoughttobeaparalogueofIL一15arisenfromgeneduplicationInChapter3，wegaveourinvestigationintothemolecularcloningtissueexpressionandprokaryoticexpressionofTetraodonIL2andIL一2LgenesIL2Lisanov

8、el4ahelixcytokinewhichreportedforthefirsttimeanditspecificallyexistinsomebonyfishesAsanothercopyofthecommonancestralgenesharingwithIL2andIL一2L，weperformtheanalysisintotheirevolutionaryrelationshipsandthemolecularevolutio