幾種優(yōu)質(zhì)產(chǎn)油真菌的篩選及發(fā)酵工藝研究.pdf

1、南京林業(yè)大學(xué)碩士學(xué)位論文幾種優(yōu)質(zhì)產(chǎn)油真菌的篩選及發(fā)酵工藝研究姓名：耿青偉申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：吳小芹李紀元20090601Studyonthescreeningandfermentationtechnologyofseveralhigh—yieldLipidfungiAbstractAsakindofnewoilresourcewithgoodprospectand，microbialoilshasbeingares

2、earchfocusInthisstudy，apreliminaryselectiononlipidproducingcapabilityoftwelvefungiwasmadeAccordingtothelipidscontentsandextractioneffectoffattyacidcomposition，asimpleandefficientmethodwasdetermined，onthisbasisofwhich，wes

3、tudiedthefermentationtechnologyoflipidwithtwofungiwhichhadbeenselectedTheresultswereasfollows：Thelipidscontents0tMisabellinaandMramannianawere2425％and2166％separately，thelipidscontentofMracemosuswas1874％Therewere三starkeyi

4、andRglutiniswhichlipidscontentswere1907％and1801％，andtheotherspecieswerealllowerthan11％GaschromatographyanalysisshowsthatthelipidacidsoffourofthemaremainlyC16C1swhichhavethemaximumcontentofoleicacidandC16～C18contentsareov

5、er90％UsingMisabellinaandLstarkeyiastheteststrainstheextractioneffectoflipidbythermalacidmethod，soxhletextractionmethodandorganicsolventmethodwereanalyzedcomparativelyTheresultsshowthatthermalacidmethodwasasimpleandefncie

6、ntmethodwhichhaveexcellenteffectonextractionofC16～C18OrganicS01ventextractionwaslessefficientOnthebasisofthesinglefactorexperimentandorthogonaldesigntheOptimizationoffermentation0tMisabellinaandLstarkeyiwerestudiedUndert

7、heoptimumcultureconditionsthebiomassyieldswere20259／Land16359／Lseparatelythelipidyieldswere9829／Land4949／Lseparatelyandthelipidcontentswere4849％and3021％separatelyKeywords：Oleaginousfungi；Mortierellaisabellina；Lipomycesst