植酸酶基因工程菌E-22的發(fā)酵工藝研究.pdf

1、Y937471攀位代碼l壁垂3墨學號2絲女3窆西韻。峽謦碩士學位論文植酸酶基因工程菌E22的發(fā)酵工藝研究論文睪者：何鎘果指導教VS彭遠義(教授)學科專業(yè)：微生物學研究方向：次生代謝與調控搌交論文日期：2006年5胃日論文答辯日期：2006年6月日學位授予單位：西南大學中國重慶2006年5月StudyontheFermentationTechnicsofGeneticEngineeringPhytaseStrainE22Disciplin

2、e：MicrobiologySupervisor：PengYuanyiCandidate：HeXigaoAbstract：Phytaseisakindofbiologicalenzyme，whichcandecompoundphyticacid(phytate)andthenreleasetheinorganicphosphorus，inositolandprotein，aminoacid，mieroelement，combinedwi

3、thphyticacid，etcThephytaseresearchstartsfromthe20thcentury60’S，andnowenteredtothemolecularlevelManykindsofmicrobialsourceofphytasehavealreadyseparatedandpurifiedMoreandmorephytasegeneswereclonedformdifferentmicroorganism

4、sAtpresent，studiesonphytasemainlyfocusonreconstructingphytasegene，changephytasecharactersuchasheatresistance，suitablepH，catalyzeactivity，etc，andimpmveitsutilizationefficiencyinfeedOtherimportanttlIingistoclonephytasegene

5、，thenhighlyeffectiveexpressioninbiologicalreactorSoitcallsolvethecontentofthephytaseistoolowinthenaturalmaterialtobemassivelyextractOurlaboratoryhasconstructedageneticengineeringphytasestrainE22Itshereditarystabilityande

6、nzymecharactercaIlwellmeetproductionsneedOnthebasisofstudiesinOurlaboratoryfollowingworkWasdone：1Theeffectofdifferentfermentationcondition：Theinoculatingtime36h，inoculatingamount10‰multiplicationtime72h，fermentationfluid

7、pH60，methanolsupply15％，amountofliquidinfermentationpot10％andtimeofinducementandexpressionof120hcanhighlyimproveproductionofphytase1644167U／mLofthephytasewasobtainedItwashigher15％thanbeforeWhenthefermentationmediumWaSadde

8、dpenicillin，Streptomycin，Triton114andTween80Ithadn’tanypromotingeffectinsteadhadsomecertaininhibitoryaction2ThestudyonsuitablecarbonSOUrceandsupplyamountsuggestedthatphraseCanachieve96067U／mLand83458u，mLrespectivelywhenU

9、Se1％glycerineand10％maltjuiceindividuallyInadditionsuitablenitrogensourceisstudiedPhytasecouldbeobtained96557U／mLwithaccession5％fishpowderandadditional5％peptone119008UtmLWasobtainedConsideringthem虹i!tionsoklrceandcost，weu

10、sed10％ofthemaltjuices粘e材bonsourceandabout5％offishpowderasthenitrogensourceTheotherfermentationconditiondidnotchange103275U／mLofphytasewasobtainedItindicatedthatisagoodresolution3Referencedtooptimizedfermentationcondition