重組馬鈴薯X病毒載體誘導煙草γ-tubulin基因沉默.pdf

1、首都師范大學碩士學位論文重組馬鈴薯X病毒載體誘導煙草γtubulin基因沉默姓名：王琦申請學位級別：碩士專業(yè)：細胞生物學指導教師：李艷紅20060520首都師范大學碩士學位論文Abstract7tubulinisaimportanceunitofmicrotubuleorganizationcenterdispersedincytoplasm、nucleolusandallmicrotubulearrays7tubulinalsopla

2、yavitalroleinmicrotubulenucleation、cellcycleandtheformationofspindleForthedisruptionof7tubulincausingtheyoungplantdeath，itbringsomediffcultsinexploringitsfunctionsInourexperiments，therecombinationPVXvectors，includingthed

3、ifferentfragmentof7tubullneDNAwereusedtoinducetheendogenesis7tubulinmRNAdegradationAndthespecialphenotyeswereexpectedforstudyingthefunctionsofTtubulinThevectorsofPVX—DandPVX—EwereselectedforinoculationtobaccosAfter14days

4、ofpostinoculation，themosaicdiseasespotswereappearedinleavesAfter2monthsofpostinoculation，thesmall—narrowandsunken—brinkleaveswerefoundDuringthetobaccodevelopment，morespecialphenotypessuchasthealternationofsmallnarrowands

5、unken—brinkleaves、stembranchingintheregionofrootandapexandthesteriletobaccosalsofoundintobaccosUsingsomemethodssuchasSEM、IFandRealtimePCR，allthephenotypesofthattobaccoswereseperatelystudyinthetissue、cellandtranscriptionl

6、evelsTheresultsshowedthatthemRNAofY～tubuliⅡintobaccoleaveweresuccessfullyknockdownbyVIGS，thelowermRNAofv—tubulinintobaccoleavecellscausedthecellsofsporgyparenchymamorebiggerandthelosssmallcellandthelowerchloroplastinpali

7、sadeparenchyma：thecytoskeletonandthedistributionofchloroplastinguardcellswerechanged：thenlllearmembranediffusedandnucleusbecamesmaller：theactivationofpollenoftobaccosdegradedAllfactsdemonstratedthatthelowerexpressionofth