截型苜蓿生物數(shù)據(jù)分析平臺的構建和耐鹽相關基因的預測與驗證.pdf

1、Y938327分類號搟級單位代碼：10019學號：s030900飛蛾囊弋式等’碩士學位論文截型苜蓿生物數(shù)據(jù)分析平臺的構建和耐鹽相關基因的預測與驗證Medicagotrunct鋤ulaBioDataAnalysisPlatformConsmlctionandSalttoleranceRelativeGenesPredictionandValidation研究生：趔亟釜指導教師：王洼數(shù)援——合作指導教師：繭震教援申請學位門類級別：理堂亟號業(yè)

2、名稱：生物絲堂生盆王生物堂研究方同：植物遺佳工程所在學院：生物堂院2006年6月AbstractThesmalldiploidgenome，selfpollinated，fastgenerationtimeandeaseoftransformationmakeMtruncatulaasagoodmodelplantforlegumegeneticsgenomicsSalinesoildistributewidelyinpasturing

3、areainchina，Studyingthemolecularmechanismsunderlyingsalt—stressenvironmentbenefittoincreaseagriculturalyield，improvesoilqualityandunderstandgeneregulation，signaltransduction，iontransponandmineralnutritioninstressenvironm

4、entsSequencingisunderwaytherearelargenumbersofESTswhicharedifficulttouseeffectivelyandlittlewebresourcesforMf，uncatulainChinaTobetterusingMtruncatulaESTsdataandotherpublicresources，facilitateinformationcommunicationandsh

5、aring，accuratestudyingspeedforkeygenesrespondingtosaltstress，weconstructaMtruncatulabio—dataanalysisplatform，called‘MedicagoTruncatulaHome”，whichintegratesMTClone，ainsilicoclonesystemspeciallyforMtruncatula，andlarge—scal

6、epredictMtruncatulagenesrelativetosalt—toleranceusingpublicresourceandMicroarraydataWehavecompletedfunctionsinbasicinformationsearch，MTClone，TCclassifyandpredictedgenesearchOurdatabasecontains36，878TC，201，653EST，650predi

7、ctedgenesrelativetosaltstress，514ArabidopsisgenesandrelatedpublicationsWeclassify650TCto7subsetsaccordingtodifferenceinrespondingtosaltstressTodeterminethevalidityoftheinsilicoanalysis，9TCwasselectedfrom3subsetstodomolec

8、ularexperimentNowWehavegot3cDNAclones，theyareTC94828，TCl06344andTCl01805Furthermore，TC94828wasinsertedintoPET30AandwehavegotprokaryoticexpressionThroughbioinformaticsanalysis，reducevalidationsequencescope，decreaseexperim