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1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文小鼠孤雌胚中細(xì)胞骨架的動(dòng)態(tài)變化與作用姓名：蘇靜艷申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：嚴(yán)云勤20050601DynamicChangesandRolesoftheCytoskeletonInMouseParthenotesAbstractMeioticmaturationandfertilizationofmammalianoocytesjsanimportantandcomplexprocess，thes

2、tudiesofooeytesparthenogeneticactivationandthedynamicchangesofcytoskeletonduringoocytesactivationwillmakeusgetadeeperunderstandingofthemechanismofthisprocessRecentyears，therearcsomereportsaboutthecytoskeletaldistribution

3、androlesofparthenotes，butthedynamicchangesofcytoskeletonintheparthenotesactivatedbyvariousstimuliarestillaproblemlackingsystematicstudiesTodate，thereareonlyafewintemalreportsaboutthisfieldThisexperimentaimedtosystematica

4、llyinvestigateandanalyzetheethanolactivatedratesofmouseoocyles，affectedfactorsofoocytesactivation，typesanddevelopmentofparthenotes，andtheorganizationandrolesofthecytoskeletonduringpronuclearformationandmigrationandearlye

5、mbryocleavagebyethanolactivationofoocytesembryocultureinvj帥andimmunofluorescencecyto—chemistryTheltsldIts：(1)(2)(3)Theethanolactivationofmouseoocytasshowedthattheactivatedanddevelopmentalratesofoocytesincreasedsignifican

6、tlywiththeincreaseofethanolconcentrationandextensionofexposuretime，butoverconcentrationandexposuretimewouldresultinincreasedfragmentratessignificantly7％ethanoltreatedoogytesfor7minwagtheoptimumactivatedconditionWiththein

7、creasesofooeyteagesandtemperature，theoocy_tesactivationanddevelopmentratesincreased，whilefmgmentratesincreasedsignificantlyOvariancumulushadnoobviouseffectontheaetivatiotlofooeytesinvitroOocytesofsexualmaturityhadbettera

8、ctivatedeffectthanundersexualmaturitymouseDegradedproportionofooeytesfirstpolarbody(PBl)andnumbersofooc歸swithoutPB1bothincreasedwiththeincreasesofoocyteagesafterhCGinjectionWithorwithoutPBlhadnosignificanteffectsontheact

9、ivation，developmentandfragmentratesWealsoobservedthatoocyteswithorwithoutPBIhadtheSalTledistributionpatternsofcytoskeletonOurfluorescenceobservationresultsindicatedthatmicmtubuleswererestrictedtotheregionofthemetaphase11

10、(MIDmeioticspindleWiththeextrusionofthesecondpolarbody(PB2)，Mllspindlebegantodisintegrateanddisappearslowlyandthecompactmicrotubulecontainingstructureknownasthemidbodywasfom]edThemidbodylocatedinthecontactsurfacesofcells

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