HL-CMS不育候選基因orf216的表達(dá)分析和煙草sm-Ngt1超表達(dá)對(duì)水稻生長(zhǎng)的影響.pdf

1、中南民族大學(xué)碩士學(xué)位論文HL-CMS不育候選基因orf216的表達(dá)分析和煙草sm-Ngt1超表達(dá)對(duì)水稻生長(zhǎng)的影響姓名：王巍葳申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：劉學(xué)群2011-05-25HL-CMS 不育候選基因 orf216 和煙草 sm-Ngt1 超表達(dá)對(duì)水稻生長(zhǎng)的影響 IIAbstract Honglian cytoplasmic male sterility (HL-CMS) is one of the thr

2、ee major CMS types in rice (Oryza sativa L.) used in commercial production of hybrid rice. There are two described candidate genes, orfH79 and orf216, involved in HL-CMS, but their sterile-induced mechanisms are still un

3、known. Glycosyltransferases (GTs) are involved in the biosynthesis and modification of many substances, and it plays a critical role in the construction of organism cell, cell metabolism and regulation of life activities

4、. In our laboratory, a new tobacco glycosyltransferase gene, sm-Ngt1, has been cloned. It is demonstrated that its expression could be dually induced by methyl jasmonate (MeJA) and salicylic acid (SA), but its physiologi

5、cal function in vivo is still unclear. In order to verify the function, the copy of orf216 in male sterile line YTA, maintainer line YTB, restorer line 9311, hybrid HL-2 and transgenic plant of orf216, and the expression

6、 analysis of HL-CMS candidates gene orf216 in RNA level were studied. On the other hand, the gene sm-Ngt1 which can lead to tobacco dwarf was transformed into the YTB (Oryza sativa L.) to see whether it can produce dwarf

7、 phenotype. 1. Using the gene orf216 as a probe, Southern blot on DNA digested with different restriction enzymes of YTA, YTB, HL-2, 9311 and transgenic plants was carried out. It is demenstrated that a single copy of or

8、f216 exists only in YTA, HL-2 and the positive transgenic plants. 2. In order to study the expression of candidate gene orf216, Northern blot on RNA extracted from anther of YTA, YTB, HL-2 and 9311 was analyzed. It was s

9、howed that the orf216 expression was detected only in male sterile line YTA, and not in HL-2. 3. By the ORF of sm-Ngt1 recombined pCAMBIA1305 vector, the sm-Ngt1 expression vector, PZ46, was constructed (Wang Zhuo, 2008)