毛細管液相色譜-高效電脈微分離方法研究.pdf

1、復旦大學碩士學位論文毛細管液相色譜高效電脈微分離方法研究姓名：黃爽申請學位級別：碩士專業(yè)：分析化學指導教師：張祥民2000.6.1一～蘭蘭查蘭竺圭蘭蘭蘭查～————————————————————————一————————————一一ABSTRACTThethesisaimsatdevelopingacomprehensivetwo—dimensionalsystemincouplingcapillaryhighperformancel

2、iquidchromatography(HPLC)withcapillaryelectrophoresis(CE)Meanwhile，relatedmethodsforcapillarycolumnpreparationsandhighspeedm／cellarelctrokineticcapillaryelectrophoresis(MEKC)weredevelopedThenewmethodshavebeenappliedtothe

3、analysisofcomplexsamplesoftraditionalChinesemedicinesandproteins，andprovetobeofextremelyhighefficientresolutioncapabilitiesThethesisisdividedinto3sectionsSinceminaturizationandhighefficiencyisageneraltrendinchromatograPh

4、ysciences，advancedstudiesinthisfieldhavebeensummarizedinthefirstsectionofthethesisMinaturizedtechniqesofcapillarygaschromatographycapillaryHPLC，CEandmicro—chipseparationwerediscussedindetailsTheprincipleofmulti—dimension

5、alseparationhasalsobeenintroduced，especiallyforHPLC—CEtechniquesInthesecondpartofthethesis，studies0112DcapillaryHPLC—CEsystem，preparationofcapillaryHPLCcolumnandtheoreticalmodesofhighspeedMEKChavebeenintensivelyexploredA

6、soneofthekeystepstoimplementthe2Dseparationsystem，one—steppreparationofOil—columnflitforcapillarycolumnusingsol—geltechnologywasdevelopedThefritischaracterizedbyresistingpackingpressureupto500barandextremepHconditionsof0

7、1mol／LHCIandNaOHpocessinggoodpermeabilityThethereticalplateheightofcapillarycolumnmaintains13—20“munderconsecutiveyearsoffrequentrunHighspeedMEKCseparationswerealsoinvestigatedThetheoreticalaspectsonplatesperunittimeVSel