混合菌固體及液體發(fā)酵鳳眼蓮產菌體蛋白飼料的研究.pdf

1、華中農業(yè)大學碩士學位論文混合菌固體及液體發(fā)酵鳳眼蓮產菌體蛋白飼料的研究姓名：賈坤申請學位級別：碩士專業(yè)：基礎獸醫(yī)學指導教師：龍良啟201106華中農業(yè)大學2010屆碩士學位論文STUDYOFMⅨEDBACTERIUMSOLIDANDLIQUIDFERMENTATIoNⅥ，ALTERHYACINTHPRODUCEDBACTERIAIPRoTEINFEEDAbstractAlongwimtheeconomicdevelopmentandpe

2、ople’Slivingstandardsimproving，people’Sdietstructureisalsoundergoingsignificantchange，peoplearetakingmoreandmoremeateggsandmilkisintheirdailylifeThiscontributedtotherapiddevelopmentofChinaslivestockbutnOWtheshortageofthe

3、feedrawmaterialshasbecomeanimportantfactorrestrictingthedevelopmentofChina’SanimalhusbandryespeciallytheproteinfeedThegoaloftheresearchisusingthemixedfermentationofwaterhyacinthtoproducesinglecellprotein，andfindingaeffec

4、tivewayofcomprehensiveutilizationofwaterhyacinthTheresearchincludethefollowing：1Thecompositionanalysisofwaterhyacinth：Webakedthefreshwaterhyacinthfor30minutesat80℃，andthencompletelydriedat65℃，grindedmillW池40mesh，andmeasu

5、reditsmainingredientsWegettheresults：crudeproteinl32％，crudefiber251％，40％crudefat，ash186％2Strainsselectingandthedeterminationofthebestcombinationofbacteriaandinoculationtoproportion：SixstainsofVhideandtwoyeastswerescreene

6、dinSolidandliquidfermentation，bymeasuringcrudeproteinyieldtheViridestrain6，yeaststrainY100andanAspergillusnigerstrainwereselectedasthebeststrainsinfermentationWechoosedthemixedfermentationofthreestrainsanddeterminedtheva

7、ccinationratioof3：2：3byexperimentTheAspergillusnigerwasvaccinatedfirstand24hoursaftertheVtrideandtheUtilisyeastwerevaccinated3TheoptimizationofmediumandcultureconditionsinsolidfermentationInthisexpedmenttherightamountofs

8、ugarwasaddedtoacceleratethegrowthrateoffungiandimprovethefermentationefficiencyWedeterminedtheratiooforganiccarbonandorganicnitrogen13：4，theamountofammoniumsulfate029，theamountofsucrose39，Ⅱlemoisturecontent60％ultimatelyb