真菌紫杉醇的分離純化和產(chǎn)物鑒定.pdf

1、南開大學(xué)碩士學(xué)位論文真菌紫杉醇的分離純化和產(chǎn)物鑒定姓名：苗會娟申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：呂憲禹朱旭東20090601ABSTRACTAbstractFungitaxolenjoysapromisingmarketbecausefungicallbegeneticallymodifiedandsustainableHowevertheirfermentationbrotharefullofimpuritiesa

2、ndhavealargeviscositySOtheProductioncostsincreaseFirstly，weoptimizedtheconditionsofhJ【ghliquidperformancechromatography(HPLC)whichwasusedtoseparatetaxolfromothersTheresultsshowedthattheoptimizedmobilephasewasmethanolwate

3、r70：30(v／v)，thetemperatureWas35℃，thespeedWas10ml／mirtandwithanUVspectrophotometermonitoredatwavelengthof227nlnfortax01Linearcalibrationgraphs)=22171073x35009wereobtainedfortaxolovertheconcentrationrangeof10l鱸IlL～01mg／mL麗

4、tllagoodcorrelation(r=09992，n_6)ThelimitofdetectionWas01肛g／ml(thesignal—tonoiseratiois2)Solidphaseextraction(SPE)technologyiswidelyusedforpretreatmentofhighliquidperformancechromatographyTwokindsofC18，YWGandFuji，andbasic

5、A1203werestudiedwithrecoveryrateasparameterWealsooptimizedandimprovedtheconditionsforelutionTheprocessofC18SPEWasactivation、sampleload、waterelution、methanol—water(v／v)20：80elutionandtaxolelution、析thmethanolwater(v／v)80：2

6、0AndtheresultsalsoshowedthatFujihadabetterdatathanYWGfor565t0447％WhilebasicA1203hadrecoveryrateofmorethan100％Whenctaorofo衄Wasusedtoelutetaxolfivetimesfromthebroth，therateWas113％；whenaceticesterwasusedonthesamewayforthree

7、times，thedataWas136％ThatmaybecausedbycatalyticabilityofbasicA1203，whichCancatalyzeanaloguesoftaxolintotaxol，forexample7EpitaxolorDABBesides，thebasicA1203wasonlyonestepelutionSOitconsumedlesssolutionandtimeAndthecollectio