苦參堿對雄激素依賴性前列腺癌細(xì)胞株LNCaP的抑制作用.pdf

1、華中科技大學(xué)博士學(xué)位論文苦參堿對雄激素依賴性前列腺癌細(xì)胞株LNCaP的抑制作用姓名：諶科申請學(xué)位級別：博士專業(yè)：外科學(xué)（泌尿外）指導(dǎo)教師：葉章群;胡志全20090501華 中 科 技 大 學(xué) 博 士 學(xué) 位 論 文 華 中 科 技 大 學(xué) 博 士 學(xué) 位 論 文 4Inhibitory Effect of Matrine on Androgen dependent Prostate Cancer Cell Line LNCaP

2、 in vitro Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Doctoral candidate: Chen ke Doctoral Advisor: Prof. Ye Zhangqun; Prof. Hu Zhiquan Abstract Purpose:

3、 To study the effect of matrine on the apoptosis and expression of AR and PSA in prostate cancer cells LNCaP. Methods: After being treated with 0.5~3.0g/L matrine for 12~36 h, the growth activities of cancer cells were s

4、tudied by MTT colorimetry and trypan blue stain assay. Cell cycle phases and apoptosis were inspected by flowcy-tometery (FCM). The levels of Bcl-2 and Bax were tested by Western Botting. The Levels of AR were tested by

5、Western Botting. The expression of prostate specific antigen (PSA) was measured by AXSYM system-chemical luciferase methods. Results: Matrine could effectively inhibit the in vitro growth of androgen dependent prostate c

6、ancer cell line LNCaP in time-and-dose-dependent manners (P <0.01). Cell cycles were arrested at G2/M phase (P<0.01).Partial cancer cells presented apoptosis (P <0.05). Matrine could decrease in the levels of Bc

7、l-2 and increase in the levels of Bax in cell line LNCaP in a dose-dependent manner (P<0.01). Matrine could decrease in the levels of AR in cell line LNCaP in a dose-dependent manner (P <0.01). Matrine inhibited th

8、e expression of PSA on prostate cancer cells (P <0.05). Conclusions: Matrine could suppress the growth of LNCaP, and promotes their apoptosis. Matrine also inhibited the expression of AR and PSA on prostate cancer cel