γ干擾素對HT29結(jié)腸癌細(xì)胞株的增殖抑制作用及對癌基因表達(dá)的影響.pdf

1、四川大學(xué)碩士學(xué)位論文γ干擾素對HT29結(jié)腸癌細(xì)胞株的增殖抑制作用及對癌基因表達(dá)的影響姓名：靳淑黎申請學(xué)位級別：碩士專業(yè)：內(nèi)科學(xué)指導(dǎo)教師：王一平20030508Y干擾素對ttT29結(jié)腸癌繃艟株的增越擲制作用及對癌基必表達(dá)的影響TheeffectofinterfenongammaonthegrowthinhibitionoftheHT29coloniccarcinomacelllineandregulatingexpressionofonc

2、ogenesproteinAbstractObjective：Theaimofthisstudywastodeterminetheeffectofinterfenongamma0171thegrowthinhibitionoftheHT29coloniccarcinomacelllineanditspossiblemechanisminvolvedMethod：MTTcolorimetry“wasusedtodeterminethesu

3、ppressingproliferationofHT29celltinebyintertbrongmnmaImmtmohistochemistrywasusedtoobservethemorphologiclocationoftumorrelatedgeneexpressingproteinincludingc—myc、bcl一2andbaxFloweyto礅戳rywasusedtoquantitivelyinvestigatethee

4、ffectofinterferongammaonHT29celllineininducingapoptosisandregulatingexpressionofproteinstatedaboveResults：InterferongammacouldinhibittheproliferationofHT29ceillineinatimedependentmannerandinduceapoptosiswhenitsconcentrat

5、ionreached100U／m1Aftertreatmentwithinterferongammafor24、48、72hours，theinhibitingratereached13％、32％、44％respectively，Immunohistochemistryshowedc—mycmainlyexpressedinkaryonwhilebel一2andbaxmainlyexpressedincytoplasm。Flowcyto